Postprandial expression of growth-related genes in Atlantic salmon (Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation

We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon (Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12 degrees C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle...

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Bibliographic Details
Published in:British Journal of Nutrition
Main Authors: Valente, Luisa M. P., Bower, Neil I., Johnston, Ian A.
Format: Article in Journal/Newspaper
Language:English
Published: 2012
Subjects:
Salmo salar L
Myogenesis
Refeeding and fasting
Nutrient availability
Insulin-like growth factor system
TROUT ONCORHYNCHUS-MYKISS
MESSENGER-RNA EXPRESSION
GILTHEAD SEA BREAM
RAINBOW-TROUT
COMPENSATORY GROWTH
SKELETAL-MUSCLE
FACTOR-I
IGF-I
SIGNALING PATHWAYS
REGULATORY FACTORS
Atlantic salmon
Salmo salar
Online Access:https://research-portal.st-andrews.ac.uk/en/researchoutput/postprandial-expression-of-growthrelated-genes-in-atlantic-salmon-salmo-salar-l-juveniles-fasted-for-1-week-and-fed-a-single-meal-to-satiation(d34194c1-f31b-4cac-a13a-17b1c19f105f).html
https://doi.org/10.1017/S0007114512000396
Description
Summary:We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon (Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12 degrees C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using quantitative real-time PCR at the following postprandial time points: -12, 0, 1, 3, 6, 12, 24, 48 and 96 h. The gut was fullest 1-6 h after feeding and emptied within 48-96 h. IGF-I, MyoD1c, MRF4 and myf5 transcripts were sharply up-regulated within 1 h of refeeding and are promising candidate genes involved in a fast-response signalling system that regulates fish myotomal muscle growth. These genes clustered together with MyoD1b and suggest a coordinated regulation to favour resumption of myogenesis as an early response to feeding. Insulin-like growth factor (IGF)-II and the ubiquitin ligase MAFbx/atrogin-1 were initially down-regulated but restored to initial values after 12 h. It is also suggested that local production of IGF-I within the muscle might suppress catabolic pathways depressing MAFbx/atrogin-1.

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