A comparative investigation of the effect of ploidy on rainbow trout gastrointestinal health
Aquaculture is the fastest growing food producing industry and demand is expected to increase with the growing global population. Thus, there is also a growing demand for farmed high value salmonids such as Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). Production of triploid...
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| Format: | Thesis |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | http://eprints.nottingham.ac.uk/67275/ https://eprints.nottingham.ac.uk/67275/1/Thesis%20Drinkall%20Corrected%20submission.pdf |
| Summary: | Aquaculture is the fastest growing food producing industry and demand is expected to increase with the growing global population. Thus, there is also a growing demand for farmed high value salmonids such as Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). Production of triploid trout is preferred by commercial fish farms, primarily because they grow larger and quicker than their diploid counterparts and their sterility ensures the prevention of breeding with wild populations in the event of escape. The epithelial surface of the intestine is a primary site of nutrient absorption, and the mucosal barrier provides protection from mechanical insult as well as forming the interface between the host microbiota, host immune system and potential pathogens. However, little is known about the effect of ploidy on the immunocompetence, function and resident microbiota of the gastrointestinal tract. This study examined the intestinal tract of a cohort of healthy juvenile triploid and diploid rainbow trout, reared under identical conditions, and studied the histo-morphological of the GI tract as well as teleost-specific TLR and mucin gene identification and expression, to better understand the role of ploidy in intestinal function and health. Through a multimodal bioinformatics investigation putative sequences of TLR18 and TLR21 were identified for the first time in the rainbow trout genome as well as a complete sequence of putative TLR19. Expression of TLR2, TLR18, TLR19 and TLR21 in both diploid and triploid intestinal tissues was confirmed by qPCR and suggested that ploidy state had little impact on relative gene expression. Appropriate reference genes were determined as part of this comparison. Existing algorithms for identification of TLRs through Leucine Rich Repeat (LRR) domains, were found to be inadequate for complete identification in teleost sequences, likely due to the presence of atypical amino acids in the conserved regions. These atypical LRRs were often categorised into the previously ... |
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