Comparison of the systemic vs. local application of Bleomicin in dogs undergoing electrochemotherapy

• Published in: Revista Científica de la Facultad de Ciencias Veterinarias
• Main Authors: Salazar-Acuña, Edison Gustavo, Castillo-Hidalgo, Edy, Moran-Obando, Wiliam
• Format: Article in Journal/Newspaper
• Language: Spanish
• Published: Universidad del Zulia p-ISSN 0798-2259; e-ISSN 2521-9715; l-ISSN 2477-944X 2022
• Subjects: Electrochemotherapy; bleomycin; local; systemic; oncology; Electroquimioterapia; bleomicina; sistémica; oncología; Alta; Quito; Canis lupus
• Online Access: https://www.produccioncientificaluz.org/index.php/cientifica/article/view/38681; https://doi.org/10.52973/rcfcv-e32140

The objective of the present study was to compare the effect of electrochemotherapy (ECT) plus local and systemic bleomycin, in dogs with neoplasms of different cellular origins, which were treated at the “La Primavera” Veterinary Clinic in the City of Quito, Pichincha, Ecuador. There were 10 canine patients (Canis lupus familiaris). The diagnosis was made by histopathology and cytology, the volumetric calculation of the tumor was obtained using the formula to calculate the tumor volume [(a×b×c×(π/6)]. The treatment was based on the administration of bleomycin 15 International Unit per square meter (IU·m2) of body surface, systemic route, and intratumoral infiltration of bleomycin at a dose of 0.25 IU per cubic centimeter (IU·cm3) of tumor surface. Considering the high selectivity of bleomycin for epithelial cells and the characteristics of tumor vascularization, electrical pulses were subsequently applied with an intensity of 1,000 volts per square centimeter (V·cm2), using different types of electrodes. Twenty eight days after the application of the therapy, the tumor masses were reduced by 50%, achieving an objective response to the applied treatment. El objetivo del presente estudio fue comparar el efecto de la electroquimioterapia (EQT) más bleomicina local y sistémica, en perros con neoplasias de diferentes orígenes celulares, los cuales fueron atendidos en la Clínica Veterinaria “La Primavera” de la ciudad de Quito, Pichincha, Ecuador, para lo cual se contó con 10 pacientes caninos (Canis lupus familiaris). El diagnostico se realizó mediante histopatología y citología, el cálculo volumétrico del tumor se obtuvo mediante la fórmula para calcular el volumen tumoral [(a×b×c×(π/6)]. El tratamiento se basó en la administración de bleomicina 15 unidades internacionales por metro cuadrado (UI·m2) de superficie corporal, vía sistémica, e infiltración intratumoral de bleomicina a la dosis de 0,25 UI por centimetro cúbico (UI·cm3) de superficie tumoral, considerando la alta selectividad de la bleomicina por las ...