The detectability of Atlantic salmon (Salmo salar) microsatellite and mitochondrial environmental DNA
Little is known about what information can be gathered from microsatellite eDNA. It is important to gain a deeper understanding of the detectability and analysis of microsatellite eDNA because it could provide information about population size that mitochondrial eDNA cannot. Water samples were colle...
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| Format: | Text |
| Language: | English |
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Scholarship@Western
2023
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| Online Access: | https://ir.lib.uwo.ca/etd/9686 https://ir.lib.uwo.ca/context/etd/article/12406/viewcontent/Simone.Miklosi.MSc.Thesis.Septmeber.20.23.pdf |
| Summary: | Little is known about what information can be gathered from microsatellite eDNA. It is important to gain a deeper understanding of the detectability and analysis of microsatellite eDNA because it could provide information about population size that mitochondrial eDNA cannot. Water samples were collected from tank and river experiments, and rivers known to contain Atlantic salmon (Salmo salar) and analyzed for Atlantic salmon mitochondrial and microsatellite eDNA. Mitochondrial eDNA was detected from all the tank experiments and 10 out of 15 rivers known to contain Atlantic salmon. Microsatellite eDNA was detected from all the tank experiments and none of the river experiments. The microsatellite alleles detected from the water were not solely representative of the genotypes of the fish in the tanks, thus individuals could not be clearly identified. The inconsistent detectability of nonrepresentative microsatellite alleles from water suggests that microsatellite eDNA cannot presently provide population-level information about Atlantic salmon. |
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