Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from Antarctic desert soil

The cold deserts of the McMurdo Dry Valleys, South Victoria Land, Eastern Antarctica, are widely acknowledged as having the harshest soil environments on Earth (6, 8, 26). Despite the apparent hostility of the environment, we and others have reported both unexpectedly high biomass (9) and phylogenet...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Heath, Caroline, Hu, Xiao Ping, Cary, Craig
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2009
Subjects:
Online Access:http://hdl.handle.net/10566/8979
https://doi.org/10.1128/AEM.02597-08
Description
Summary:The cold deserts of the McMurdo Dry Valleys, South Victoria Land, Eastern Antarctica, are widely acknowledged as having the harshest soil environments on Earth (6, 8, 26). Despite the apparent hostility of the environment, we and others have reported both unexpectedly high biomass (9) and phylogenetic diversity (1, 19, 24, 29) in Antarctic soils. The presence of numerous novel taxa suggests that these soils might prove to be valuable sources of genetic material for mining novel industrial enzymes active at low temperatures (9, 23). Esterases (EC 3.1.1.1) and lipases (EC 3.1.1.3) catalyze the hydrolysis and synthesis of ester compounds. Their applications in industry cover a broad spectrum, including as detergent additives, in food processing, in environmental bioremediation, and in biomass and plant waste degradation for the production of useful organocompounds (3, 16).