Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome

A novel esterase was identified through functional screening of an Antarctic desert soil metagenomic library in E. coli. The 297-amino acid sequence had only low (< 29 %) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7-54 °C and...

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Published in:Applied and Environmental Microbiology
Main Authors: Health, Caroline, Hu, Xiao Ping, Cary, S. Craig, Cowan, Don A.
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2009
Subjects:
Antarctic desert
soil metagenome
Antarctic
Antarc*
Online Access:https://hdl.handle.net/10289/5382
https://doi.org/10.1128/AEM.02597-08
id ftunivwaikato:oai:researchcommons.waikato.ac.nz:10289/5382
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spelling ftunivwaikato:oai:researchcommons.waikato.ac.nz:10289/5382 2024-02-11T09:56:55+01:00 Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome Health, Caroline Hu, Xiao Ping Cary, S. Craig Cowan, Don A. United States 2009 https://hdl.handle.net/10289/5382 https://doi.org/10.1128/AEM.02597-08 en eng American Society for Microbiology http://aem.asm.org/cgi/content/short/AEM.02597-08v1 Applied Environmental Microbiology Health, C., Hu, X.P., Cary, S.C. & Cowan, D. (2009). Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome. Applied and Environmental Microbiology, 77, 1153-1161. https://hdl.handle.net/10289/5382 doi:10.1128/AEM.02597-08 Antarctic desert soil metagenome Journal Article 2009 ftunivwaikato https://doi.org/10.1128/AEM.02597-08 2024-01-23T18:25:20Z A novel esterase was identified through functional screening of an Antarctic desert soil metagenomic library in E. coli. The 297-amino acid sequence had only low (< 29 %) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7-54 °C and at alkaline pH levels, and is a potential candidate for industrial application. Article in Journal/Newspaper Antarc* Antarctic The University of Waikato: Research Commons Antarctic Applied and Environmental Microbiology 75 13 4657 4659
institution Open Polar
collection The University of Waikato: Research Commons
op_collection_id ftunivwaikato
language English
topic Antarctic desert
soil metagenome
spellingShingle Antarctic desert
soil metagenome
Health, Caroline
Hu, Xiao Ping
Cary, S. Craig
Cowan, Don A.
Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome
topic_facet Antarctic desert
soil metagenome
description A novel esterase was identified through functional screening of an Antarctic desert soil metagenomic library in E. coli. The 297-amino acid sequence had only low (< 29 %) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7-54 °C and at alkaline pH levels, and is a potential candidate for industrial application.
format Article in Journal/Newspaper
author Health, Caroline
Hu, Xiao Ping
Cary, S. Craig
Cowan, Don A.
author_facet Health, Caroline
Hu, Xiao Ping
Cary, S. Craig
Cowan, Don A.
author_sort Health, Caroline
title Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome
title_short Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome
title_full Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome
title_fullStr Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome
title_full_unstemmed Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome
title_sort isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an antarctic desert soil metagenome
publisher American Society for Microbiology
publishDate 2009
url https://hdl.handle.net/10289/5382
https://doi.org/10.1128/AEM.02597-08
op_coverage United States
geographic Antarctic
geographic_facet Antarctic
genre Antarc*
Antarctic
genre_facet Antarc*
Antarctic
op_relation http://aem.asm.org/cgi/content/short/AEM.02597-08v1
Applied Environmental Microbiology
Health, C., Hu, X.P., Cary, S.C. & Cowan, D. (2009). Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome. Applied and Environmental Microbiology, 77, 1153-1161.
https://hdl.handle.net/10289/5382
doi:10.1128/AEM.02597-08
op_doi https://doi.org/10.1128/AEM.02597-08
container_title Applied and Environmental Microbiology
container_volume 75
container_issue 13
container_start_page 4657
op_container_end_page 4659
_version_ 1790606939128856576

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