Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome

A novel esterase was identified through functional screening of an Antarctic desert soil metagenomic library in E. coli. The 297-amino acid sequence had only low (< 29 %) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7-54 °C and...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Health, Caroline, Hu, Xiao Ping, Cary, S. Craig, Cowan, Don A.
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 2009
Subjects:
Antarctic desert
soil metagenome
Antarctic
Antarc*
Online Access:https://hdl.handle.net/10289/5382
https://doi.org/10.1128/AEM.02597-08
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Summary:A novel esterase was identified through functional screening of an Antarctic desert soil metagenomic library in E. coli. The 297-amino acid sequence had only low (< 29 %) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7-54 °C and at alkaline pH levels, and is a potential candidate for industrial application.

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