Isolation and characterisation of a novel, low-temperature-1 active alkaliphilic esterase from an Antarctic desert soil metagenome
A novel esterase was identified through functional screening of an Antarctic desert soil metagenomic library in E. coli. The 297-amino acid sequence had only low (< 29 %) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7-54 °C and...
Published in: | Applied and Environmental Microbiology |
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Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
2009
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Subjects: | |
Online Access: | https://hdl.handle.net/10289/5382 https://doi.org/10.1128/AEM.02597-08 |
Summary: | A novel esterase was identified through functional screening of an Antarctic desert soil metagenomic library in E. coli. The 297-amino acid sequence had only low (< 29 %) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7-54 °C and at alkaline pH levels, and is a potential candidate for industrial application. |
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