Differences between intestinal segments and soybean meal-induced changes in intestinal mucus composition of common carp Cyprinus carpio L.

The alimentary tract is a possible site where pathogens and toxins can enter. The alimentary tract is protected, amongst others, by mucus. In this study, tissue samples and crude mucus preparations from different parts of the intestinal tract of Cyprinus carpio (from intestinal bulb onto the hindgut...

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Bibliographic Details
Published in:Aquaculture Nutrition
Main Authors: van der Marel, M.C., Propsting, M.J., Battermann, F., Jung-Schroers, V., Hubner, A., Rombout, J.H.W.M., Steinhagen, D.
Format: Article in Journal/Newspaper
Language:English
Published: 2014
Subjects:
adhesion
aeromonas-hydrophila
atlantic salmon
distal intestine
in-vivo
induced enteritis
mucin glycoproteins
perorally applied endotoxin
salmon salmo-salar
skin mucus
Atlantic salmon
Salmo salar
Online Access:https://research.wur.nl/en/publications/differences-between-intestinal-segments-and-soybean-meal-induced-
https://doi.org/10.1111/anu.12041
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Summary:The alimentary tract is a possible site where pathogens and toxins can enter. The alimentary tract is protected, amongst others, by mucus. In this study, tissue samples and crude mucus preparations from different parts of the intestinal tract of Cyprinus carpio (from intestinal bulb onto the hindgut) were examined using histological, histochemical and biochemical techniques. Furthermore, the response of the intestinal mucosal layer and intestinal mucus of C. carpio to acute soybean meal (SBM)-induced enteritis was investigated. In the present study, an indication for a different protein core of mucus high molecular weight glycoproteins (HMGs) for first and second segment could not be found. However, differences in mucus glycosylation could be found. Along the gut axis, the size of the major protein peaks were not similar, which can be caused by a different glycosylation. Also, differences in staining for the antimicrobial peptide beta-defensin 2 were found. Furthermore, changes in HMGs upon SBM diet were found similar to those found in inflammatory bowel disease (IBD) in humans. Initial changes include: changes in mucin composition, the presence of BD3 and of bacteria in internal organs. After the initial changes, all values measured returned back to the initial pre-SBM diet values.

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