Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules

The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC–MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontol...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Rajan, B., Fernandes, J.M.O., Caipang, C.M.A., Kiron, V., Rombout, J.H.W.M., Brinchmann, M.
Format: Article in Journal/Newspaper
Language:English
Published: 2011
Subjects:
binding-lectin
channel catfish
conger-myriaster
cyclophilin-a
cyprinus-carpio
epidermal mucus
ictalurus-punctatus
intestinal mucus
lepeophtheirus-salmonis
neutrophil elastase
atlantic cod
Gadus morhua
Online Access:https://research.wur.nl/en/publications/proteome-reference-map-of-the-skin-mucus-of-atlantic-cod-gadus-mo
https://doi.org/10.1016/j.fsi.2011.05.006
Description
Summary:The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC–MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR

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