Bioassay-derived dioxin equivalent concentrations in gonads and livers of the Atlantic cod females from the Baltic Sea

The DR-H4IIE.Luc bioassay is based on the ability of dioxin and dioxin-like contaminants to activate the AhR and its signal transduction pathway, a mechanism through which these contaminants elicit their toxic effects. The bioassay was used to examine the total dioxin-equivalent (TEQ) toxicity in go...

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Bibliographic Details
Published in:Ecotoxicology and Environmental Safety
Main Authors: Dabrowska, H., Murk, A.J., van den Berg, J.H.J.
Format: Article in Journal/Newspaper
Language:English
Published: 2010
Subjects:
active compounds
aromatic-hydrocarbons
biphenyls
calux bioassay
dibenzo-p-dioxins
dr-calux(r) bioassay
expression calux
fish
in-vitro bioassay
sediments
atlantic cod
Online Access:https://research.wur.nl/en/publications/bioassay-derived-dioxin-equivalent-concentrations-in-gonads-and-l
https://doi.org/10.1016/j.ecoenv.2010.08.030
Description
Summary:The DR-H4IIE.Luc bioassay is based on the ability of dioxin and dioxin-like contaminants to activate the AhR and its signal transduction pathway, a mechanism through which these contaminants elicit their toxic effects. The bioassay was used to examine the total dioxin-equivalent (TEQ) toxicity in gonads and livers of cod females from the southern Baltic Sea. The bioassay-derived TEQ-luc was measured after 24-h and 48-h exposure periods. Mean concentrations in the 24-h bioassay were 95 and 35 pg TEQ-luc g-1 lipid in gonads and livers, respectively, and 58 and 38 pg TEQ-luc g-1 lipid in the 48-h bioassay, respectively. The 48-h TEQ-luc levels displayed significant relationships with SPCB7 and selected PCB congeners but not with the TEQDLPCB-REP. Levels in gonads approached 10% of the LC50 for developing larvae of other marine fish, yet the impact on survival of the cod during its early life remains to be assessed in a future

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