Resistance of common carp (Cyprinus carpio L.) to Cyprinid herpesvirus-3 is influenced by major histocompatibility (MH) class II B gene polymorphism

• Published in: Fish & Shellfish Immunology
• Main Authors: Rakus, K.L., Wiegertjes, G.F., Adamek, M., Siwicki, A.K., Lepa, A., Irnazarow, I.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2009
• Subjects: aeromonas-salmonicida; atlantic salmon; cd4(+) t-cells; complex loci; disease resistance; frequency-dependent selection; gill necrosis virus; interstitial nephritis; koi herpesvirus; salmon salmo-salar; Atlantic salmon; Salmo salar
• Online Access: https://research.wur.nl/en/publications/resistance-of-common-carp-cyprinus-carpio-l-to-cyprinid-herpesvir; https://doi.org/10.1016/j.fsi.2009.03.001

The role of MH class II B (Cyca-DAB1-like) genes in resistance of common carp (Cyprinus carpio L) to Cyprinid herpesvirus-3 (CyHV-3), also known as koi herpesvirus (KHV) was analysed. The material consisted of 934 fish from six carp crosses. Fish were challenged with CyHV-3 at an age of 7 and 10 months. During challenge experiments the peak of mortality caused by CyHV-3 was observed at days 8-12 p.i. and the overall cumulative mortality reached 79.9%. Among six Cyca-DAB1-like genotypes, revealed by PCR-RF-SSCP analysis, one genotype (E) was found associated with higher resistance to CyHV-3. Three other genotypes (B, H and J) could be linked to higher susceptibility to CyHV-3. Analysis of the alleles that compose the Cyca-DAB1-like genotypes linked one particular allele (Cyca-DAB1*05) to significantly increased, and two alleles (Cyca-DAB1*02 and Cyca-DAB1*06) to significantly decreased resistance to CyHV-3. Our data indicate that MH class II B genes could be used as potential genetic markers in breeding of common carp for resistance to this virus.