Choline dihydrogen phosphate-based deep eutectic solvent: a suitable bioplatform for lipase extraction

• Published in: Separation and Purification Technology
• Main Authors: Álvarez Álvarez, María Salomé, Rivas Soilán, Miguel Ángel, Longo González, María Asunción, Deive Herva, Francisco Javier, Rodríguez Rodríguez, Ana María
• Format: Article in Journal/Newspaper
• Language: English
• Published: Separation and Purification Technology 2021
• Subjects: 2213.09 Equilibrios Termodinámico; 2302.09 Enzimología; Triton; Bancroft; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/11093/2921; https://doi.org/10.1016/j.seppur.2021.118525

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUG In this work, we have demonstrated the suitability of a novel Deep Eutectic Solvent (DES) composed of cholinium dihydrogen phosphate and ethylene glycol at a molar ratio of 1:2 (ChDHP:EG) to extract Candida antarctica lipase B from aqueous solutions of non-ionic surfactants Triton X-100 and Tween 80. The binodal curves were firstly explored at temperatures between 298.15 and 333.15 K and the experimental data were correlated by means of empirical models based on exponential and polynomial equations. The tie-lines have been ascertained by means of density and refractive indices measurement, and Othmer-Tobias, Bancroft and Setschenow equations were proposed to correlate these data. The Tween 80-based Aqueous Biphasic System was demonstrated to avoid any deleterious effect on the lipase biocatalytic activity, and more than 80% of the enzyme activity was retained on the DES-rich phase, which proves the suitability of these platforms to be employed in downstream stages in lipase production processes. Xunta de Galicia | Ref. ED481D-2019/017 Xunta de Galicia | Ref. ED431F 2016/007 Agencia Estatal de Investigación | Ref. RTI2018-094702-B-I00