Satellitome analysis of the Pacific Oyster Crassostrea gigas reveals new pattern of satellite DNA organization, highly scattered across the genome

Several features already qualified the invasive bivalve species Crassostrea gigas as a valuable non-standard model organism in genome research. C. gigas is characterized by the low contribution of satellite DNAs (satDNAs) vs. mobile elements and has an extremely low amount of heterochromatin, predom...

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Bibliographic Details
Published in:International Journal of Molecular Sciences
Main Authors: Tunjić Cvitanić, Monika, Pasantes Ludeña, Juan José, García Souto, Daniel, Cvitanić, Tonči, Plohl, Miroslav, Šatović Vukšić, Eva
Format: Article in Journal/Newspaper
Language:English
Published: International Journal of Molecular Sciences 2021
Subjects:
2401.08 Genética Animal
2409.03 Genética de Poblaciones
2407.02 Citogenética
Pacific
Crassostrea gigas
Pacific oyster
Online Access:http://hdl.handle.net/11093/2977
https://doi.org/10.3390/ijms22136798
https://www.mdpi.com/1422-0067/22/13/6798
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Summary:Several features already qualified the invasive bivalve species Crassostrea gigas as a valuable non-standard model organism in genome research. C. gigas is characterized by the low contribution of satellite DNAs (satDNAs) vs. mobile elements and has an extremely low amount of heterochromatin, predominantly built of DNA transposons. In this work, we have identified 52 satDNAs composing the satellitome of C. gigas and constituting about 6.33% of the genome. Satellitome analysis reveals unusual, highly scattered organization of relatively short satDNA arrays across the whole genome. However, peculiar chromosomal distribution and densities are specific for each satDNA. The inspection of the organizational forms of the 11 most abundant satDNAs shows association with constitutive parts of Helitron mobile elements. Nine of the inspected satDNAs are dominantly found in mobile element-associated form, two mostly appear standalone, and only one is present exclusively as Helitron-associated sequence. The Helitron-related satDNAs appear in more chromosomes than other satDNAs, indicating that these mobile elements could be leading satDNA propagation in C. gigas. No significant accumulation of satDNAs on certain chromosomal positions was detected in C. gigas, thus establishing a novel pattern of satDNA organization on the genome level. Hrvatska Zaklada za Znanost | Ref. IP-2019-045522 Xunta de Galicia | Ref. ED481B/2018/091 Xunta de Galicia | Ref. ED431C 2020/05

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