Synthesis and characterization of a lipase-friendly DES based on cholinium dihydrogen phosphate

• Published in: Journal of Molecular Liquids
• Main Authors: Álvarez Álvarez, María Salomé, Longo González, María Asunción, Deive Herva, Francisco Javier, Rodríguez Rodríguez, Ana María
• Format: Article in Journal/Newspaper
• Language: English
• Published: Journal of Molecular Liquids 2021
• Subjects: 2301.18 Métodos Termoanalíticos; 2301.09 Espectroscopia de Resonancia Magnética; 2302.09 Enzimología; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/11093/2970; https://doi.org/10.1016/j.molliq.2021.117230; https://linkinghub.elsevier.com/retrieve/pii/S0167732221019541

The main objective of the present work was to synthesize and characterize three novel lipase-friendly deep eutectic solvents (DESs) using cholinium dihydrogen phosphate (N1112OHDHP) as hydrogen bond acceptor and ethylene glycol (EG) and/or glycerol (GL) as hydrogen bond donors in the following proportions: N1112OHDHP:EG (molar ratio 1:2), N1112OHDHP:GL (molar ratio 1:2) and N1112OHDHP:EG:GL (molar ratio 1:1:1). FTIR, 1H NMR, TGA and DSC were employed to shed light on their distinctive structural characteristics. Density, refractive index and dynamic viscosity were also measured at different temperatures, and the experimental data were correlated with several well-known equations. Finally, the suitability of the newly synthesized DESs to be employed in biocatalytic reactions was demonstrated by analysing the activity of a commercial enzyme (Candida antarctica lipase B) after being in contact with them. Financiado para publicación en acceso aberto: Universidade de Vigo/CISUG Agencia Estatal de Investigación | Ref. RTI2018-094702-B-I00 Xunta de Galicia | Ref. ED481D-2019/017