Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)

Published version, source at http://doi.org/10.3732/apps.1500036 . License in accordance with the journal's policy - CC-BY-NC-SA . Premise of the study: Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaul...

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Bibliographic Details
Published in:Applications in Plant Sciences
Main Authors: Müller, Eike, Hlavackova, Iva, Svoen, Mildrid Elvik, Alsos, Inger Greve, Eidesen, Pernille Bronken
Format: Article in Journal/Newspaper
Language:English
Published: Botanical Society of America 2015
Subjects:
Arctic/alpine
Caryophyllaceae
gynodioecious
moss campion
Silene acaulis
VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480
Arctic
Moss campion
Online Access:https://hdl.handle.net/10037/8881
https://doi.org/10.3732/apps.1500036
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Summary:Published version, source at http://doi.org/10.3732/apps.1500036 . License in accordance with the journal's policy - CC-BY-NC-SA . Premise of the study: Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaulis (Caryophyllaceae). Methods and Results: Fourteen out of 50 markers resulted in polymorphic products with profiles that enabled interpretation. The numbers of alleles per locus ranged from two to six, and the expected heterozygosity per locus ranged from 0.06 to 0.68. Analysis of F0 and F1 samples proved that one allele was always inherited maternally. Four multiplex mixes have been developed. Conclusions: Microsatellite markers for this species will be a valuable tool to study detailed small-scale genetic patterns in an arctic/alpine herb and to relate them to demographic parameters.

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