Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA

Background: A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96). Resul...

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Bibliographic Details
Published in:BMC Microbiology
Main Authors: Henningsson, Anna J., Hvidsten, Dag, Kristiansen, Bjørn Erik, Matussek, Andreas, Stuen, Snorre, Jenkins, Andrew
Format: Article in Journal/Newspaper
Language:English
Published: BioMed Central 2015
Subjects:
Anaplasma phagocytophilum
Ixodes ricinus
TaqMan realtime PCR
Norway
gltA
Prevalence
VDP::Mathematics and natural science: 400::Basic biosciences: 470
VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470
Arctic
Northern Norway
Online Access:https://hdl.handle.net/10037/8076
https://doi.org/10.1186/s12866-015-0486-5
Description
Summary:Background: A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96). Results: Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % (p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. Conclusion: This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway.

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