Hydrocarbon biodegradation potential in environmental bacterial metagenome

• Main Author: Caro Pascual, Alicia
• Format: Master Thesis
• Language: English
• Published: UiT Norges arktiske universitet 2020
• Subjects: Microbiology; Bioremediation; Metagenome; hydrocarbons; VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472; VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472; VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecotoxicology: 489; VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økotoksikologi: 489; BIO-3950; Barents Sea; Murmansk; Norway; Kola Bay
• Online Access: https://hdl.handle.net/10037/20579

Hydrocarbon pollution in marine environments present an acute problem which is aggravated by cold temperatures. This is especially relevant in important environmental and economic northern regions such as the Barents Sea. The Barents Sea region has become the focus of oil industries from Russia and Norway, incrementing the risk of hydrocarbon pollution. Bioremediation is a cost-effective and environmentally sound method to remove hydrocarbon pollution. To study the bioremediation potential of native bacteria from a chronically oil polluted region of the Barents Sea a study was done in the Murmansk seaport (Kola Bay, 68°58′00′′ N, 33°05′00′′ E) analyzing the composition of the bacterial community. This present work aims to do a deeper study of that bacterial community through a metagenomic fosmid library, a selective hydrocarbon-rich medium and bioinformatic analysis. A metagenomic fosmid library was constructed with the environmental DNA, the fosmid clones were transformed in Escherichia coli cells and cultivated in minimal media with concentrations 0.05%, 0.5% and 1% of diesel or crude oil. Ten colonies were selected, sequenced, and subsequently analyzed with the software Geneious 2020.2.2. BLAST searches and other bioinformatic tools were conducted in every Open Reading Frame of the 10 colonies, revealing new links between native bacteria and hydrocarbon degradation as well as promising enzymes for hydrocarbon bioremediation. This work sets the ground for further studies on functional metagenomic analyses with fosmid libraries and further studies with the novel bacterial species and enzymes linked to hydrocarbon biodegradation.