Hydrocarbon biodegradation potential in environmental bacterial metagenome
Hydrocarbon pollution in marine environments present an acute problem which is aggravated by cold temperatures. This is especially relevant in important environmental and economic northern regions such as the Barents Sea. The Barents Sea region has become the focus of oil industries from Russia and...
Main Author: | |
---|---|
Format: | Master Thesis |
Language: | English |
Published: |
UiT Norges arktiske universitet
2020
|
Subjects: | |
Online Access: | https://hdl.handle.net/10037/20579 |
id |
ftunivtroemsoe:oai:munin.uit.no:10037/20579 |
---|---|
record_format |
openpolar |
spelling |
ftunivtroemsoe:oai:munin.uit.no:10037/20579 2023-05-15T15:38:37+02:00 Hydrocarbon biodegradation potential in environmental bacterial metagenome Caro Pascual, Alicia 2020-12-15 https://hdl.handle.net/10037/20579 eng eng UiT Norges arktiske universitet UiT The Arctic University of Norway https://hdl.handle.net/10037/20579 openAccess Copyright 2020 The Author(s) Microbiology Bioremediation Metagenome hydrocarbons VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecotoxicology: 489 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økotoksikologi: 489 BIO-3950 Master thesis Mastergradsoppgave 2020 ftunivtroemsoe 2021-06-25T17:58:03Z Hydrocarbon pollution in marine environments present an acute problem which is aggravated by cold temperatures. This is especially relevant in important environmental and economic northern regions such as the Barents Sea. The Barents Sea region has become the focus of oil industries from Russia and Norway, incrementing the risk of hydrocarbon pollution. Bioremediation is a cost-effective and environmentally sound method to remove hydrocarbon pollution. To study the bioremediation potential of native bacteria from a chronically oil polluted region of the Barents Sea a study was done in the Murmansk seaport (Kola Bay, 68°58′00′′ N, 33°05′00′′ E) analyzing the composition of the bacterial community. This present work aims to do a deeper study of that bacterial community through a metagenomic fosmid library, a selective hydrocarbon-rich medium and bioinformatic analysis. A metagenomic fosmid library was constructed with the environmental DNA, the fosmid clones were transformed in Escherichia coli cells and cultivated in minimal media with concentrations 0.05%, 0.5% and 1% of diesel or crude oil. Ten colonies were selected, sequenced, and subsequently analyzed with the software Geneious 2020.2.2. BLAST searches and other bioinformatic tools were conducted in every Open Reading Frame of the 10 colonies, revealing new links between native bacteria and hydrocarbon degradation as well as promising enzymes for hydrocarbon bioremediation. This work sets the ground for further studies on functional metagenomic analyses with fosmid libraries and further studies with the novel bacterial species and enzymes linked to hydrocarbon biodegradation. Master Thesis Barents Sea Kola Bay University of Tromsø: Munin Open Research Archive Barents Sea Murmansk Norway |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
Microbiology Bioremediation Metagenome hydrocarbons VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecotoxicology: 489 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økotoksikologi: 489 BIO-3950 |
spellingShingle |
Microbiology Bioremediation Metagenome hydrocarbons VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecotoxicology: 489 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økotoksikologi: 489 BIO-3950 Caro Pascual, Alicia Hydrocarbon biodegradation potential in environmental bacterial metagenome |
topic_facet |
Microbiology Bioremediation Metagenome hydrocarbons VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Ecotoxicology: 489 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økotoksikologi: 489 BIO-3950 |
description |
Hydrocarbon pollution in marine environments present an acute problem which is aggravated by cold temperatures. This is especially relevant in important environmental and economic northern regions such as the Barents Sea. The Barents Sea region has become the focus of oil industries from Russia and Norway, incrementing the risk of hydrocarbon pollution. Bioremediation is a cost-effective and environmentally sound method to remove hydrocarbon pollution. To study the bioremediation potential of native bacteria from a chronically oil polluted region of the Barents Sea a study was done in the Murmansk seaport (Kola Bay, 68°58′00′′ N, 33°05′00′′ E) analyzing the composition of the bacterial community. This present work aims to do a deeper study of that bacterial community through a metagenomic fosmid library, a selective hydrocarbon-rich medium and bioinformatic analysis. A metagenomic fosmid library was constructed with the environmental DNA, the fosmid clones were transformed in Escherichia coli cells and cultivated in minimal media with concentrations 0.05%, 0.5% and 1% of diesel or crude oil. Ten colonies were selected, sequenced, and subsequently analyzed with the software Geneious 2020.2.2. BLAST searches and other bioinformatic tools were conducted in every Open Reading Frame of the 10 colonies, revealing new links between native bacteria and hydrocarbon degradation as well as promising enzymes for hydrocarbon bioremediation. This work sets the ground for further studies on functional metagenomic analyses with fosmid libraries and further studies with the novel bacterial species and enzymes linked to hydrocarbon biodegradation. |
format |
Master Thesis |
author |
Caro Pascual, Alicia |
author_facet |
Caro Pascual, Alicia |
author_sort |
Caro Pascual, Alicia |
title |
Hydrocarbon biodegradation potential in environmental bacterial metagenome |
title_short |
Hydrocarbon biodegradation potential in environmental bacterial metagenome |
title_full |
Hydrocarbon biodegradation potential in environmental bacterial metagenome |
title_fullStr |
Hydrocarbon biodegradation potential in environmental bacterial metagenome |
title_full_unstemmed |
Hydrocarbon biodegradation potential in environmental bacterial metagenome |
title_sort |
hydrocarbon biodegradation potential in environmental bacterial metagenome |
publisher |
UiT Norges arktiske universitet |
publishDate |
2020 |
url |
https://hdl.handle.net/10037/20579 |
geographic |
Barents Sea Murmansk Norway |
geographic_facet |
Barents Sea Murmansk Norway |
genre |
Barents Sea Kola Bay |
genre_facet |
Barents Sea Kola Bay |
op_relation |
https://hdl.handle.net/10037/20579 |
op_rights |
openAccess Copyright 2020 The Author(s) |
_version_ |
1766369773766049792 |