Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site

• Published in: Standards in Genomic Sciences
• Main Authors: Wissuwa, Juliane, Stokke, Runar, Fedøy, Anita-Elin, Lian, Kjersti, Smalås, Arne O., Steen, Ida Helene
• Format: Article in Journal/Newspaper
• Language: English
• Published: BioMed Central 2016
• Subjects: VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474; VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474; Thermophile; Geobacillus; Enzymes; Bioprospecting; Arctic; Jan Mayen
• Online Access: https://hdl.handle.net/10037/10810; https://doi.org/10.1186/s40793-016-0137-y

Published version. Source at http://doi.org/10.1186/s40793-016-0137-y. License CC BY 4.0. Members of the genus Geobacillus have been isolated from a wide variety of habitats worldwide and are the subject for targeted enzyme utilization in various industrial applications. Here we report the isolation and complete genome sequence of the thermophilic starch-degrading Geobacillus sp. 12AMOR1. The strain 12AMOR1 was isolated from deep-sea hot sediment at the Jan Mayen hydrothermal Vent Site. Geobacillus sp. 12AMOR1 consists of a 3,410,035 bp circular chromosome and a 32,689 bp plasmid with a G + C content of 52 % and 47 %, respectively. The genome comprises 3323 protein-coding genes, 88 tRNA species and 10 rRNA operons. The isolate grows on a suite of sugars, complex polysaccharides and proteinous carbon sources. Accordingly, a versatility of genes encoding carbohydrate-active enzymes (CAZy) and peptidases were identified in the genome. Expression, purification and characterization of an enzyme of the glycoside hydrolase family 13 revealed a starch-degrading capacity and high thermal stability with a melting temperature of 76.4 °C. Altogether, the data obtained point to a new isolate from a marine hydrothermal vent with a large bioprospecting potential.