A DEMETER-like DNA demethylase governs tomato fruit ripening

In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DMLs). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only a few studies demonstrate the developmenta...

Full description

Bibliographic Details
Published in:Proceedings of the National Academy of Sciences
Main Authors: Liu, Ruie, How-Kit, Alexandre, Stammitti, Linda, Teyssier, Emeline, Rolin, Dominique, Mortain-Bertrand, Anne, Halle, Stefanie, Liu, Mingchun, Kong, Junhua, Wu, Chaoqun, Degraeve-Guibault, Charlotte, Chapman, Natalie H., Maucourt, Mickael, Hodgman, T. Charlie, Tost, Jörg, Bouzayen, Mondher, Hong, Yiguo, Seymour, Graham B., Giovannoni, James J., Gallusci, Philippe
Other Authors: Commissariat à l'Energie Atomique et aux énergies alternatives - CEA (FRANCE), Cornell University (USA), Institut National Polytechnique de Toulouse - INPT (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), University of Nottingham (UNITED KINGDOM), University of Warwick (UNITED KINGDOM), Fondation Jean Dausset - Centre d’Etude du Polymorphisme Humain - CEPH (FRANCE), Hangzhou Normal University - HZNU (CHINA), Université de Bordeaux (FRANCE), Laboratoire Epigénétique et Environnement - LEE (Evry,France)
Format: Article in Journal/Newspaper
Language:English
Published: National Academy of Sciences 2015
Subjects:
Biologie végétale
Génétique des plantes
Sciences agricoles
Active DNA demethylation
DNA glycosylase lyase
Epigenetic
Tomato
Fruit ripening
DML
Online Access:http://oatao.univ-toulouse.fr/14400/
http://oatao.univ-toulouse.fr/14400/7/Liu.pdf
https://doi.org/10.1073/pnas.1503362112
Description
Summary:In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DMLs). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only a few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here, we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripening— an important developmental process unique to plants. RNAi SlDML2 knockdown results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato.

Similar Items