n-Alkane and long-chain alcohol recovery in moose (Alces alces), a browsing herbivore

Habitat management for herbivores often depends on an understanding of the food habits of animals. Plant cuticular waxes containing nearly indigestible complex mixture of n-alkanes and long-chain alcohols (LCOHs) have recently shown promise for diet analyses, but the accuracy of the technique depend...

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Bibliographic Details
Main Authors: Carnahan, Anthony, Spalinger, Donald, Collins, William
Format: Article in Journal/Newspaper
Language:unknown
Published: NRC Research Press (a division of Canadian Science Publishing) 2017
Subjects:
Online Access:http://hdl.handle.net/1807/88209
http://www.nrcresearchpress.com/doi/abs/10.1139/cjz-2017-0131
Description
Summary:Habitat management for herbivores often depends on an understanding of the food habits of animals. Plant cuticular waxes containing nearly indigestible complex mixture of n-alkanes and long-chain alcohols (LCOHs) have recently shown promise for diet analyses, but the accuracy of the technique depends strongly on the efficiency of recovery of the markers in feces. Fecal recovery of n-alkanes and LCOHs from ten browse stems or leaves and two ensiled grass hays fed to moose (Alces alces (L., 1758)) during in vivo digestion trials was investigated. n-Alkanes and LCOHs were extracted using a single-step accelerated solvent extraction technique, and the recovery of these cuticular components was calculated from the feces of the animals. n-Alkane recoveries from feces averaged 0.82, but ranged from a low of 0.58 (haylage) to a high of 0.95 (browse stems). Long-chain alcohol recoveries averaged 0.92 across all forages, ranging from 0.80 (haylage) to a high of 1.13 (browse stems). n-Alkane and LCOH fecal recovery increased with increasing chain length, similar to findings in other studies. Although fecal recovery of n-alkanes and LCOHs were variable, we conclude that they are inversely related to forage digestibility, are consistent within forage classes, and are therefore predictable markers for use in assessing herbivore diets. The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author.