DNASER. I. Layout and data analysis

We present the DNA analyzer (DNASER), a novel bioinstrumentation for real-time acquisition and elaboration of images from fluorescent DNA microarrays. A white light beam illuminates the target sample allowing the images grabbing on a high sensibility and wide-band charge-coupled device camera (ORCA...

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Bibliographic Details
Main Authors: Nicolini C., Malvezzi A. M., Tomaselli A., Sposito D., Tropiano G., BORGOGNO MONDINO, ENRICO CORRADO
Other Authors: Malvezzi A.M., Borgogno E.
Format: Article in Journal/Newspaper
Language:English
Published: 2002
Subjects:
Orca
Online Access:http://hdl.handle.net/2318/118333
Description
Summary:We present the DNA analyzer (DNASER), a novel bioinstrumentation for real-time acquisition and elaboration of images from fluorescent DNA microarrays. A white light beam illuminates the target sample allowing the images grabbing on a high sensibility and wide-band charge-coupled device camera (ORCA II-Hamamatsu). This high-performance device permits to acquire images faster and of higher quality than the traditional systems. The DNA microarrays images are processed to recognize the DNA chip spots, to analyze their superficial distribution on the glass slide and to evaluate their geometric and intensity properties. Differently form conventional techniques, the spots analysis is fully automated and the DNASER does not require any additional information about the DNA microarray geometry. The DNASER hardware and software architecture is illustrated. Preliminary results are shown from experiments performed on real DNA samples.

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