Evaluation of quantitative polymerase chain reaction to assess nosZ gene prevalence in mixed microbial communities

The usefulness of quantitative polymerase chain reaction (QPCR) to measure nosZ gene prevalence in a multi-template reaction was assessed by comparing 19 nosZ template DNA samples and 91 model communities. Efficiencies of the QPCR varied but were not significantly different among nosZ genotypes and...

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Bibliographic Details
Published in:Canadian Journal of Microbiology
Main Authors: Sicilliano, SD, Wai, M, Powell, SM
Format: Article in Journal/Newspaper
Language:English
Published: NRC Research Press 2007
Subjects:
270307 Microbial Ecology
denitrification
nosZ
quantitative PCR
microbial ecology
Antarctic
Arctic
soils
Antarc*
Online Access:https://eprints.utas.edu.au/4107/
https://eprints.utas.edu.au/4107/1/4107.pdf
https://doi.org/10.1139/W07-014
Description
Summary:The usefulness of quantitative polymerase chain reaction (QPCR) to measure nosZ gene prevalence in a multi-template reaction was assessed by comparing 19 nosZ template DNA samples and 91 model communities. Efficiencies of the QPCR varied but were not significantly different among nosZ genotypes and were not linked to genetic distance from Ralstonia eutropha. nosZ genotype QPCR efficiencies obtained from isolated denitrifiers were higher (84.8%) than those obtained from excised denaturing gradient gel electrophoresis bands or clones of PCR products from total community DNA (ca. 60%). Analysis of the model communities indicated that QPCR accurately predicts gene prevalence in communities composed of up to six templates.

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