Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L
Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inocu...
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Online Access: | https://doi.org/10.1016/j.fsi.2013.12.013 http://www.ncbi.nlm.nih.gov/pubmed/24378682 http://ecite.utas.edu.au/91201 |
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ftunivtasecite:oai:ecite.utas.edu.au:91201 2023-05-15T15:30:58+02:00 Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L Pennacchi, Y Leef, MJ Crosbie, PBB Nowak, BF Bridle, AR 2014 https://doi.org/10.1016/j.fsi.2013.12.013 http://www.ncbi.nlm.nih.gov/pubmed/24378682 http://ecite.utas.edu.au/91201 en eng Elsevier http://dx.doi.org/10.1016/j.fsi.2013.12.013 Pennacchi, Y and Leef, MJ and Crosbie, PBB and Nowak, BF and Bridle, AR, Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L, Fish and Shellfish Immunology, 36, (2) pp. 563-570. ISSN 1050-4648 (2014) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/24378682 http://ecite.utas.edu.au/91201 Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Refereed Article PeerReviewed 2014 ftunivtasecite https://doi.org/10.1016/j.fsi.2013.12.013 2019-12-13T21:54:26Z Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inoculation using 2D quantitative RT-PCR, a method of mapping transcriptional responses in tissues. The genes of interest were IL-1β, TNF-α, TCR-α chain, CD8, CD4, MHC-IIα, MHC-I, IgM and IgT. A significant increase in expression of the mRNA of all the genes was observed in the gills of AGD-affected fish. Contrary to previous studies, our data suggest that the parasite, N.perurans , elicits a classical inflammatory response in the gills of AGD-affected fish and indicates that the mRNA expression of immune genes within gill lesions misrepresents the cellular immune response in the gills during AGD. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Fish & Shellfish Immunology 36 2 563 570 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
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Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture Pennacchi, Y Leef, MJ Crosbie, PBB Nowak, BF Bridle, AR Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Aquaculture |
description |
Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inoculation using 2D quantitative RT-PCR, a method of mapping transcriptional responses in tissues. The genes of interest were IL-1β, TNF-α, TCR-α chain, CD8, CD4, MHC-IIα, MHC-I, IgM and IgT. A significant increase in expression of the mRNA of all the genes was observed in the gills of AGD-affected fish. Contrary to previous studies, our data suggest that the parasite, N.perurans , elicits a classical inflammatory response in the gills of AGD-affected fish and indicates that the mRNA expression of immune genes within gill lesions misrepresents the cellular immune response in the gills during AGD. |
format |
Article in Journal/Newspaper |
author |
Pennacchi, Y Leef, MJ Crosbie, PBB Nowak, BF Bridle, AR |
author_facet |
Pennacchi, Y Leef, MJ Crosbie, PBB Nowak, BF Bridle, AR |
author_sort |
Pennacchi, Y |
title |
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L |
title_short |
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L |
title_full |
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L |
title_fullStr |
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L |
title_full_unstemmed |
Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L |
title_sort |
evidence of immune and inflammatory processes in the gills of agd-affected atlantic salmon, salmo salar l |
publisher |
Elsevier |
publishDate |
2014 |
url |
https://doi.org/10.1016/j.fsi.2013.12.013 http://www.ncbi.nlm.nih.gov/pubmed/24378682 http://ecite.utas.edu.au/91201 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1016/j.fsi.2013.12.013 Pennacchi, Y and Leef, MJ and Crosbie, PBB and Nowak, BF and Bridle, AR, Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L, Fish and Shellfish Immunology, 36, (2) pp. 563-570. ISSN 1050-4648 (2014) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/24378682 http://ecite.utas.edu.au/91201 |
op_doi |
https://doi.org/10.1016/j.fsi.2013.12.013 |
container_title |
Fish & Shellfish Immunology |
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36 |
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2 |
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563 |
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570 |
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1766361459748503552 |