Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L

Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inocu...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Pennacchi, Y, Leef, MJ, Crosbie, PBB, Nowak, BF, Bridle, AR
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2014
Subjects:
Agricultural and Veterinary Sciences
Fisheries Sciences
Aquaculture
Atlantic salmon
Salmo salar
Online Access:https://doi.org/10.1016/j.fsi.2013.12.013
http://www.ncbi.nlm.nih.gov/pubmed/24378682
http://ecite.utas.edu.au/91201
Description
Summary:Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inoculation using 2D quantitative RT-PCR, a method of mapping transcriptional responses in tissues. The genes of interest were IL-1β, TNF-α, TCR-α chain, CD8, CD4, MHC-IIα, MHC-I, IgM and IgT. A significant increase in expression of the mRNA of all the genes was observed in the gills of AGD-affected fish. Contrary to previous studies, our data suggest that the parasite, N.perurans , elicits a classical inflammatory response in the gills of AGD-affected fish and indicates that the mRNA expression of immune genes within gill lesions misrepresents the cellular immune response in the gills during AGD.

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