Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L
The use of swabs relative to organs as a sample collection method for the detection of Tasmanian salmon reovirus (TSRV) in farmed Tasmanian Atlantic salmon, Salmo salar L., was evaluated by RT-qPCR. Evaluation of individual and pooled sample collection (organs vs swabs) was carried out to determine...
Published in: | Journal of Fish Diseases |
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Language: | English |
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Blackwell Publishing Ltd
2013
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Online Access: | https://doi.org/10.1111/jfd.12012 http://www.ncbi.nlm.nih.gov/pubmed/23121165 http://ecite.utas.edu.au/86380 |
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ftunivtasecite:oai:ecite.utas.edu.au:86380 2023-05-15T15:30:58+02:00 Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L Zainathan, SC Carson, J Crane, MSJ Nowak, BF 2013 https://doi.org/10.1111/jfd.12012 http://www.ncbi.nlm.nih.gov/pubmed/23121165 http://ecite.utas.edu.au/86380 en eng Blackwell Publishing Ltd http://dx.doi.org/10.1111/jfd.12012 Zainathan, SC and Carson, J and Crane, MSJ and Nowak, BF, Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L, Journal of Fish Diseases, 36, (4) pp. 427-436. ISSN 0140-7775 (2013) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/23121165 http://ecite.utas.edu.au/86380 Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases Refereed Article PeerReviewed 2013 ftunivtasecite https://doi.org/10.1111/jfd.12012 2019-12-13T21:50:27Z The use of swabs relative to organs as a sample collection method for the detection of Tasmanian salmon reovirus (TSRV) in farmed Tasmanian Atlantic salmon, Salmo salar L., was evaluated by RT-qPCR. Evaluation of individual and pooled sample collection (organs vs swabs) was carried out to determine the sensitivity of the collection methods and the effect of pooling of samples for the detection of TSRV. Detection of TSRV in individual samples was as sensitive when organs were sampled compared to swabs, and in pooled samples, organs demonstrated a sensitivity of one 10-fold dilution higher than sampling of pooled swabs. Storage of swabs at 4 C for t = 24 h demonstrated results similar to those at t = 0. Advantages of using swabs as a preferred sample collection method for the detection of TSRV compared to organ samples are evident from these experimental trials. Article in Journal/Newspaper Atlantic salmon Salmo salar eCite UTAS (University of Tasmania) Journal of Fish Diseases 36 4 427 436 |
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eCite UTAS (University of Tasmania) |
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ftunivtasecite |
language |
English |
topic |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
spellingShingle |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases Zainathan, SC Carson, J Crane, MSJ Nowak, BF Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L |
topic_facet |
Agricultural and Veterinary Sciences Fisheries Sciences Fish Pests and Diseases |
description |
The use of swabs relative to organs as a sample collection method for the detection of Tasmanian salmon reovirus (TSRV) in farmed Tasmanian Atlantic salmon, Salmo salar L., was evaluated by RT-qPCR. Evaluation of individual and pooled sample collection (organs vs swabs) was carried out to determine the sensitivity of the collection methods and the effect of pooling of samples for the detection of TSRV. Detection of TSRV in individual samples was as sensitive when organs were sampled compared to swabs, and in pooled samples, organs demonstrated a sensitivity of one 10-fold dilution higher than sampling of pooled swabs. Storage of swabs at 4 C for t = 24 h demonstrated results similar to those at t = 0. Advantages of using swabs as a preferred sample collection method for the detection of TSRV compared to organ samples are evident from these experimental trials. |
format |
Article in Journal/Newspaper |
author |
Zainathan, SC Carson, J Crane, MSJ Nowak, BF |
author_facet |
Zainathan, SC Carson, J Crane, MSJ Nowak, BF |
author_sort |
Zainathan, SC |
title |
Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L |
title_short |
Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L |
title_full |
Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L |
title_fullStr |
Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L |
title_full_unstemmed |
Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L |
title_sort |
laboratory evaluation of sample collection methods (organs vs swabs) for tasmanian salmon reovirus detection in farmed atlantic salmon, salmo salar l |
publisher |
Blackwell Publishing Ltd |
publishDate |
2013 |
url |
https://doi.org/10.1111/jfd.12012 http://www.ncbi.nlm.nih.gov/pubmed/23121165 http://ecite.utas.edu.au/86380 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://dx.doi.org/10.1111/jfd.12012 Zainathan, SC and Carson, J and Crane, MSJ and Nowak, BF, Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L, Journal of Fish Diseases, 36, (4) pp. 427-436. ISSN 0140-7775 (2013) [Refereed Article] http://www.ncbi.nlm.nih.gov/pubmed/23121165 http://ecite.utas.edu.au/86380 |
op_doi |
https://doi.org/10.1111/jfd.12012 |
container_title |
Journal of Fish Diseases |
container_volume |
36 |
container_issue |
4 |
container_start_page |
427 |
op_container_end_page |
436 |
_version_ |
1766361459588071424 |