Laboratory evaluation of sample collection methods (organs vs swabs) for Tasmanian salmon reovirus detection in farmed Atlantic salmon, Salmo salar L

The use of swabs relative to organs as a sample collection method for the detection of Tasmanian salmon reovirus (TSRV) in farmed Tasmanian Atlantic salmon, Salmo salar L., was evaluated by RT-qPCR. Evaluation of individual and pooled sample collection (organs vs swabs) was carried out to determine...

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Bibliographic Details
Published in:Journal of Fish Diseases
Main Authors: Zainathan, SC, Carson, J, Crane, MSJ, Nowak, BF
Format: Article in Journal/Newspaper
Language:English
Published: Blackwell Publishing Ltd 2013
Subjects:
Agricultural and Veterinary Sciences
Fisheries Sciences
Fish Pests and Diseases
Atlantic salmon
Salmo salar
Online Access:https://doi.org/10.1111/jfd.12012
http://www.ncbi.nlm.nih.gov/pubmed/23121165
http://ecite.utas.edu.au/86380
Description
Summary:The use of swabs relative to organs as a sample collection method for the detection of Tasmanian salmon reovirus (TSRV) in farmed Tasmanian Atlantic salmon, Salmo salar L., was evaluated by RT-qPCR. Evaluation of individual and pooled sample collection (organs vs swabs) was carried out to determine the sensitivity of the collection methods and the effect of pooling of samples for the detection of TSRV. Detection of TSRV in individual samples was as sensitive when organs were sampled compared to swabs, and in pooled samples, organs demonstrated a sensitivity of one 10-fold dilution higher than sampling of pooled swabs. Storage of swabs at 4 C for t = 24 h demonstrated results similar to those at t = 0. Advantages of using swabs as a preferred sample collection method for the detection of TSRV compared to organ samples are evident from these experimental trials.

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