Neoparamoeba perurans n. sp., an agent of amoebic gill disease of Atlantic Salmon ( Salmo salar )

Amoebic gill disease (AGD) is a potentially fatal disease of some marine fish. Two amphizoic amoebae Neoparamoeba pemaquidensis and Neoparamoeba branchiphila have been cultured from AGD-affected fish, yet it is not known if one or both are aetiological agents. Here, we PCR amplified the 18S rRNA gen...

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Bibliographic Details
Published in:International Journal for Parasitology
Main Authors: Young, ND, Crosbie, PBB, Adams, MB, Nowak, BF, Morrison, RN
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier Ltd 2007
Subjects:
Agricultural
Veterinary and Food Sciences
Fisheries sciences
Aquaculture
Atlantic salmon
Salmo salar
Online Access:https://doi.org/10.1016/j.ijpara.2007.04.018
http://www.ncbi.nlm.nih.gov/pubmed/17561022
http://ecite.utas.edu.au/48400
Description
Summary:Amoebic gill disease (AGD) is a potentially fatal disease of some marine fish. Two amphizoic amoebae Neoparamoeba pemaquidensis and Neoparamoeba branchiphila have been cultured from AGD-affected fish, yet it is not known if one or both are aetiological agents. Here, we PCR amplified the 18S rRNA gene of non-cultured, gill-derived (NCGD) amoebae from AGD-affected Atlantic salmon ( Salmo salar ) using N. pemaquidensis and N. branchiphila -specific oligonucleotides. Variability in PCR amplification led to comparisons of 18S rRNA and 28S rRNA gene sequences from NCGD and clonal cultured, gill-derived (CCGD) N. pemaquidensis and N. branchiphila . Phylogenetic analyses inferred from either 18S or 28S rRNA gene sequences unambiguously segregated a lineage consisting of NCGD amoebae from other members of the genus Neoparamoeba . Species-specific oligonucleotide probes that hybridise 18S rRNA were designed, validated and used to probe gill tissue from AGD-affected Atlantic salmon. The NCGD amoebae-specific probe bound AGD-associated amoebae while neither N. pemaquidensis nor N. branchiphila were associated with AGD-lesions. Together, these data indicate that NCGD amoebae are a new species, designated Neoparamoeba perurans n.sp. and this is the predominant aetiological agent of AGD of Atlantic salmon cultured in Tasmania, Australia.

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