Production of eicosanoids derived from 20:4n-6 and 20:5n-3 in primary cultures of turbot (Scophthalmus maximus) brain astrocytes in response to platelet activating factor, substance P and interleukin-1 beta

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
• Main Authors: Tocher, Douglas R, Bell, J Gordon, Sargent, John R
• Other Authors: Institute of Aquaculture, University of Stirling, orcid:0000-0002-8603-9410
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 1996
• Subjects: astrocytes; eicosanoids; polyunsaturated fatty acids; primary culture; Scophthalmus maximus; Turbot
• Online Access: http://hdl.handle.net/1893/7565; https://doi.org/10.1016/0305-0491(96)00113-7; http://dspace.stir.ac.uk/bitstream/1893/7565/1/tocher_compbiochemphysiol115b_1996.pdf

Primary cultures of turbot (Scophthalmus maximus) brain astroglial cells established in medium containing fetal bovine serum contain increased proportions of 18:1(n-9), total (n-9) and (n-6) polyunsaturated fatty acids (PUFA) and greatly reduced (n-3) PUFA in comparison with turbot brain. Supplementation with a mixture of 5 μM eicosapentaenoic [20:5(n-3)] and 25 μM docosahexaenoic [22:6(n-3)] acids for 4 days significantly inreased the percentages of these acids in total cellular lipid of turbot astrocytes and restored the (n-3) PUFA composition of the cells to that found in turbot brain. The production of prostaglandins (PG) E and F of the 2- and 3-series and leukotrienes (LT) C4 and C5 in response to various agonists was determined in PUFA-supplemented astrocytes. Calcium ionophore A23187, platelet activating factor and substance P stimulated the production of both PGF and PGE. Interleukin-lβ significantly stimulated the production of PGF only. There were differences between the agonists in their effects on the relative levels of 2- and 3-series PGs produced. Only very low amounts of LTC were produced by the turbot astrocytes, with only substance P showing a minor stimulatory effect.