Long noncoding RNAs (lncRNAs) dynamics evidence immunomodulation during ISAV-Infected Atlantic salmon (Salmo salar)

Despite evidence for participation in the host response to infection, the roles of many long non-coding RNAs (lncRNAs) remain unknown. Therefore, the aims of this study were to identify lncRNAs in Atlantic salmon (Salmo salar) and evaluate their transcriptomic regulation during ISA virus (ISAV) infe...

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Bibliographic Details
Published in:Scientific Reports
Main Authors: Boltana, Sebastian, Valenzuela-Miranda, Diego, Aguilar, Andrea, MacKenzie, Simon, Gallardo-Escarate, Cristian
Other Authors: University of Concepcion, Institute of Aquaculture, orcid:0000-0003-1845-6826
Format: Article in Journal/Newspaper
Language:English
Published: Nature Publishing group 2016
Subjects:
Atlantic salmon
Salmo salar
Online Access:http://hdl.handle.net/1893/23335
https://doi.org/10.1038/srep22698
http://dspace.stir.ac.uk/bitstream/1893/23335/1/srep22698.pdf
Description
Summary:Despite evidence for participation in the host response to infection, the roles of many long non-coding RNAs (lncRNAs) remain unknown. Therefore, the aims of this study were to identify lncRNAs in Atlantic salmon (Salmo salar) and evaluate their transcriptomic regulation during ISA virus (ISAV) infection, an Orthomyxoviridae virus associated with high mortalities in salmonid aquaculture. Using next-generation sequencing, whole-transcriptome analysis of theSalmo salarresponse to ISAV infection was performed, identifying 5,636 putative lncRNAs with a mean length of 695 base pairs. The transcriptional modulation evidenced a similar number of differentially expressed lncRNAs in the gills (3,294), head-kidney (3,275), and liver (3,325) over the course of the infection. Moreover, analysis of a subset of these lncRNAs showed the following: (i) Most were similarly regulated in response to ISA virus infection; (ii) The transcript subsets were uniquely modulated in each tissue (gills, liver, and head-kidney); and (iii) A subset of lncRNAs were upregulated for each tissue and time analysed, indicating potential markers for ISAV infection. These findings represent the first discovery of widespread differential expression of lncRNAs in response to virus infection in non-model species, suggesting that lncRNAs could be involved in regulating the host response during ISAV infection.

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