Development of new-tools to investigate toxicological hazard due to endocrine disruptor organochlorines and emerging contaminants in mediterranean cetaceans

The possibility that certain Mediterranean cetaceans are subject to toxicological hazard due to organochlorines and emerging contaminants, such as polybrominated diphenyl ethers (PBDEs) with endocrine disrupting capacity, was investigated using non-lethal methods. The need for new biomarkers for EDC...

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Bibliographic Details
Published in:Marine Environmental Research
Main Authors: FOSSI, M. C., MARSILI, L., CASINI, S., BUCALOSSI, D.
Other Authors: Fossi, M. C., Marsili, L., Casini, S., Bucalossi, D.
Format: Article in Journal/Newspaper
Language:English
Published: 2006
Subjects:
Mediterranean cetacean
organochlorine contaminant
brominated flame retardant
endocrine disrupting chemical
fibroblasts cell culture
immunofluorescence technique
western blot
Balaenoptera physalus
Online Access:http://hdl.handle.net/11365/39679
https://doi.org/10.1016/j.marenvres.2006.04.063
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Summary:The possibility that certain Mediterranean cetaceans are subject to toxicological hazard due to organochlorines and emerging contaminants, such as polybrominated diphenyl ethers (PBDEs) with endocrine disrupting capacity, was investigated using non-lethal methods. The need for new biomarkers for EDCs and for a "cell model" to explore the different susceptibilities to several classes of ECDs, including emerging contaminants, led us to culture fibroblasts of different cetacean species ("dolphins in test tubes"). We then explored interspecies and gender susceptibility to OGEDCs and PBDEs using qualitative and semi-quantitative evaluation of target proteins, such as CYP1A and CYP2B in cultured cetacean fibroblasts (Stenella coeruleoalba, Tursiops truncatus and Balaenoptera physalus), by western blot and immunofluorescence techniques. (c) 2006 Elsevier Ltd. All rights reserved.

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