Improved noninvasive genotyping method: application to brown bear (Ursus arctos) faeces
Times Cited: 13 International audience We redesigned new microsatellite primers and one sex-specific primer for amplification of faecal DNA from brown bears (Ursus arctos). We also combined a semi-nested polymerase chain reaction (PCR) with a newly developed multiplex preamplification method in orde...
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Online Access: | https://hal.science/halsde-00279779 https://doi.org/10.1111/j.1471-8286.2004.00711.x |
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ftunivsavoie:oai:HAL:halsde-00279779v1 2024-04-28T08:41:01+00:00 Improved noninvasive genotyping method: application to brown bear (Ursus arctos) faeces Bellemain, E. Taberlet, P. Laboratoire d'Ecologie Alpine (LECA) Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS) Department of Ecology and Natural Resource Management Norwegian University of Life Sciences (NMBU) 2004 https://hal.science/halsde-00279779 https://doi.org/10.1111/j.1471-8286.2004.00711.x en eng HAL CCSD Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-8286.2004.00711.x halsde-00279779 https://hal.science/halsde-00279779 doi:10.1111/j.1471-8286.2004.00711.x ISSN: 1471-8278 EISSN: 1471-8286 Molecular Ecology Notes https://hal.science/halsde-00279779 Molecular Ecology Notes, 2004, 4 (3), pp.519-522. ⟨10.1111/j.1471-8286.2004.00711.x⟩ microsatellites multiplex preamplification method noninvasive samples sex-specific primer Ursus arctos [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment info:eu-repo/semantics/article Journal articles 2004 ftunivsavoie https://doi.org/10.1111/j.1471-8286.2004.00711.x 2024-04-11T00:46:23Z Times Cited: 13 International audience We redesigned new microsatellite primers and one sex-specific primer for amplification of faecal DNA from brown bears (Ursus arctos). We also combined a semi-nested polymerase chain reaction (PCR) with a newly developed multiplex preamplification method in order to increase the quality of the amplified DNA fragments. In comparison with a conventional PCR approach, the genotyping error rate was substantially reduced and the amplification rate was increased. This new approach could be transposed to other species where conventional PCR methods experience low success due to limited DNA concentration and/or quality. Article in Journal/Newspaper Ursus arctos Université Savoie Mont Blanc: HAL Molecular Ecology Notes 4 3 519 522 |
institution |
Open Polar |
collection |
Université Savoie Mont Blanc: HAL |
op_collection_id |
ftunivsavoie |
language |
English |
topic |
microsatellites multiplex preamplification method noninvasive samples sex-specific primer Ursus arctos [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment |
spellingShingle |
microsatellites multiplex preamplification method noninvasive samples sex-specific primer Ursus arctos [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment Bellemain, E. Taberlet, P. Improved noninvasive genotyping method: application to brown bear (Ursus arctos) faeces |
topic_facet |
microsatellites multiplex preamplification method noninvasive samples sex-specific primer Ursus arctos [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment |
description |
Times Cited: 13 International audience We redesigned new microsatellite primers and one sex-specific primer for amplification of faecal DNA from brown bears (Ursus arctos). We also combined a semi-nested polymerase chain reaction (PCR) with a newly developed multiplex preamplification method in order to increase the quality of the amplified DNA fragments. In comparison with a conventional PCR approach, the genotyping error rate was substantially reduced and the amplification rate was increased. This new approach could be transposed to other species where conventional PCR methods experience low success due to limited DNA concentration and/or quality. |
author2 |
Laboratoire d'Ecologie Alpine (LECA) Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS) Department of Ecology and Natural Resource Management Norwegian University of Life Sciences (NMBU) |
format |
Article in Journal/Newspaper |
author |
Bellemain, E. Taberlet, P. |
author_facet |
Bellemain, E. Taberlet, P. |
author_sort |
Bellemain, E. |
title |
Improved noninvasive genotyping method: application to brown bear (Ursus arctos) faeces |
title_short |
Improved noninvasive genotyping method: application to brown bear (Ursus arctos) faeces |
title_full |
Improved noninvasive genotyping method: application to brown bear (Ursus arctos) faeces |
title_fullStr |
Improved noninvasive genotyping method: application to brown bear (Ursus arctos) faeces |
title_full_unstemmed |
Improved noninvasive genotyping method: application to brown bear (Ursus arctos) faeces |
title_sort |
improved noninvasive genotyping method: application to brown bear (ursus arctos) faeces |
publisher |
HAL CCSD |
publishDate |
2004 |
url |
https://hal.science/halsde-00279779 https://doi.org/10.1111/j.1471-8286.2004.00711.x |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
ISSN: 1471-8278 EISSN: 1471-8286 Molecular Ecology Notes https://hal.science/halsde-00279779 Molecular Ecology Notes, 2004, 4 (3), pp.519-522. ⟨10.1111/j.1471-8286.2004.00711.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-8286.2004.00711.x halsde-00279779 https://hal.science/halsde-00279779 doi:10.1111/j.1471-8286.2004.00711.x |
op_doi |
https://doi.org/10.1111/j.1471-8286.2004.00711.x |
container_title |
Molecular Ecology Notes |
container_volume |
4 |
container_issue |
3 |
container_start_page |
519 |
op_container_end_page |
522 |
_version_ |
1797571442051645440 |