Improved noninvasive genotyping method: application to brown bear (Ursus arctos) faeces

Times Cited: 13 International audience We redesigned new microsatellite primers and one sex-specific primer for amplification of faecal DNA from brown bears (Ursus arctos). We also combined a semi-nested polymerase chain reaction (PCR) with a newly developed multiplex preamplification method in orde...

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Bibliographic Details
Published in:Molecular Ecology Notes
Main Authors: Bellemain, E., Taberlet, P.
Other Authors: Laboratoire d'Ecologie Alpine (LECA), Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS), Department of Ecology and Natural Resource Management, Norwegian University of Life Sciences (NMBU)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2004
Subjects:
microsatellites
multiplex preamplification method
noninvasive samples
sex-specific primer
Ursus arctos
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
[SDV.BID]Life Sciences [q-bio]/Biodiversity
[SDV.EE]Life Sciences [q-bio]/Ecology
environment
Online Access:https://hal.science/halsde-00279779
https://doi.org/10.1111/j.1471-8286.2004.00711.x
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Summary:Times Cited: 13 International audience We redesigned new microsatellite primers and one sex-specific primer for amplification of faecal DNA from brown bears (Ursus arctos). We also combined a semi-nested polymerase chain reaction (PCR) with a newly developed multiplex preamplification method in order to increase the quality of the amplified DNA fragments. In comparison with a conventional PCR approach, the genotyping error rate was substantially reduced and the amplification rate was increased. This new approach could be transposed to other species where conventional PCR methods experience low success due to limited DNA concentration and/or quality.

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