Presence of an isoform of H+-pyrophosphatase located in the alveolar sacs of a scuticociliate parasite of turbot: physiological consequences

• Published in: Parasitology
• Main Authors: Mallo Seijas, Natalia, Lamas Fernández, Jesús, Felipe González, Ana Paula de, Castro de Antonio, María Eugenia de, Sueiro Benavides, Rosa Ana, Leiro Vidal, José Manuel
• Other Authors: Universidade de Santiago de Compostela. Departamento de Bioloxía Funcional, Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía, Universidade de Santiago de Compostela. Instituto de Acuicultura, Universidade de Santiago de Compostela. Instituto de Investigación e Análises Alimentarias
• Format: Article in Journal/Newspaper
• Language: English
• Published: Cambridge University Press
• Subjects: H+-PPase; Philasterides dicentrarchi; Alveolar sacs; Osmoregulation; Turbot
• Online Access: http://hdl.handle.net/10347/18171; https://doi.org/10.1017/S0031182015001997

This article has been published in a revised form in Parasitology [http://doi.org/10.1017/ S0031182015001997]. This version is free to view and download for private research and study only. Not for re-distribution, re-sale or use in derivative works. © 2017 Cambridge University Press H+-pyrophosphatases (H+-PPases) are integral membrane proteins that couple pyrophosphate energy to an electrochemical gradient across biological membranes and promote the acidification of cellular compartments. Eukaryotic organisms, essentially plants and protozoan parasites, contain various types of H+-PPases associated with vacuoles, plasma membrane and acidic Ca+2 storage organelles called acidocalcisomes. We used Lysotracker Red DND-99 staining to identify two acidic cellular compartments in trophozoites of the marine scuticociliate parasite Philasterides dicentrarchi: the phagocytic vacuoles and the alveolar sacs. The membranes of these compartments also contain H+-PPase, which may promote acidification of these cell structures. We also demonstrated for the first time that the P. dicentrarchi H+-PPase has two isoforms: H+-PPase 1 and 2. Isoform 2, which is probably generated by splicing, is located in the membranes of the alveolar sacs and has an amino acid motif recognized by the H+-PPase-specific antibody PABHK. The amino acid sequences of different isolates of this ciliate are highly conserved. Gene and protein expression in this isoform are significantly regulated by variations in salinity, indicating a possible physiological role of this enzyme and the alveolar sacs in osmoregulation and salt tolerance in P. dicentrarchi This work was financially supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 634429 (PARAFISHCONTROL), by the Ministerio de Economía y Competitividad (Spain) under grant agreement AGL2014-57125-R and by grant GPC2014/069 from the Xunta de Galicia (Spain) SI