Construction and Annotation of a High Density SNP Linkage Map of the Atlantic Salmon (Salmo salar) Genome

• Published in: G3 Genes|Genomes|Genetics
• Main Authors: Tsai, Hsin Y., Robledo Sánchez, Diego, Lowe, Natalie R., Bekaert, Michaël, Taggart, John B., Bron, James E., Houston, Ross D.
• Other Authors: Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física
• Format: Article in Journal/Newspaper
• Language: English
• Published: Genetics Society of America
• Subjects: Salmo salar; Linkage map; SNP array; Recombination; RNA-Seq; Stirling; Atlantic salmon
• Online Access: http://hdl.handle.net/10347/16319; https://doi.org/10.1534/g3.116.029009

High density linkage maps are useful tools for fine-scale mapping of quantitative trait loci, and characterization of the recombination landscape of a species’ genome. Genomic resources for Atlantic salmon (Salmo salar) include a well-assembled reference genome, and high density single nucleotide polymorphism (SNP) arrays. Our aim was to create a high density linkage map, and to align it with the reference genome assembly. Over 96,000 SNPs were mapped and ordered on the 29 salmon linkage groups using a pedigreed population comprising 622 fish from 60 nuclear families, all genotyped with the ‘ssalar01’ high density SNP array. The number of SNPs per group showed a high positive correlation with physical chromosome length (r = 0.95). While the order of markers on the genetic and physical maps was generally consistent, areas of discrepancy were identified. Approximately 6.5% of the previously unmapped reference genome sequence was assigned to chromosomes using the linkage map. Male recombination rate was lower than females across the vast majority of the genome, but with a notable peak in subtelomeric regions. Finally, using RNA-Seq data to annotate the reference genome, the mapped SNPs were categorized according to their predicted function, including annotation of 2500 putative nonsynonymous variants. The highest density SNP linkage map for any salmonid species has been created, annotated, and integrated with the Atlantic salmon reference genome assembly. This map highlights the marked heterochiasmy of salmon, and provides a useful resource for salmonid genetics and genomics research The University of Edinburgh. Edinburgh Genomics is partly supported through core grants from the Natural Environment Research Council (NERC) (R8/H10/56), Medical Research Council (MRC) (MR/K001744/1),and the Biotechnology and Biological Sciences Research Council (BBSRC) (BB/J004243/1). This research was supported by BBSRC grants (BB/H022007/1, BB/F002750/1, BB/F001959/1) awarded to The Roslin Institute and University of Stirling, and ...