H+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the pH-sensitive dye acridine orange

Monitoring the fluorescence quenching of the pH-sensitive dye Acridine orange, proton accumulation in the presence of an inside-negative transmembrane potential was measured in eel (Anguilla anguilla) intestinal brush-border membrane vesicles. It was demonstrated that the proton accumulation was spe...

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Published in:Biochimica et Biophysica Acta (BBA) - Biomembranes
Main Authors: VERRI, Tiziano, MAFFIA, Michele, STORELLI, Carlo
Other Authors: Verri, Tiziano, Maffia, Michele, Storelli, Carlo
Format: Article in Journal/Newspaper
Language:English
Published: 1992
Subjects:
Online Access:http://hdl.handle.net/11587/108598
https://doi.org/10.1016/0005-2736(92)90303-4
http://www.sciencedirect.com/science/article/pii/0005273692903034
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spelling ftunivsalento:oai:iris.unisalento.it:11587/108598 2024-04-14T08:00:59+00:00 H+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the pH-sensitive dye acridine orange VERRI, Tiziano MAFFIA, Michele STORELLI, Carlo Verri, Tiziano Maffia, Michele Storelli, Carlo 1992 STAMPA http://hdl.handle.net/11587/108598 https://doi.org/10.1016/0005-2736(92)90303-4 http://www.sciencedirect.com/science/article/pii/0005273692903034 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:A1992JQ43000017 volume:1110 firstpage:123 lastpage:126 numberofpages:4 journal:BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES http://hdl.handle.net/11587/108598 doi:10.1016/0005-2736(92)90303-4 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0026672922 http://www.sciencedirect.com/science/article/pii/0005273692903034 Fluorescence acridine orange proton/dipeptide cotransport intestine (eel) info:eu-repo/semantics/article 1992 ftunivsalento https://doi.org/10.1016/0005-2736(92)90303-4 2024-03-21T18:05:39Z Monitoring the fluorescence quenching of the pH-sensitive dye Acridine orange, proton accumulation in the presence of an inside-negative transmembrane potential was measured in eel (Anguilla anguilla) intestinal brush-border membrane vesicles. It was demonstrated that the proton accumulation was specifically increased by the presence of the dipeptide glycyl-glycine in the extravesicular space, showing saturation kinetics at increasing dipeptide concentrations and was specifically inhibited by diethylpyrocarbonate. Data reported sugge Article in Journal/Newspaper Anguilla anguilla Università del Salento: CINECA IRIS Biochimica et Biophysica Acta (BBA) - Biomembranes 1110 1 123 126
institution Open Polar
collection Università del Salento: CINECA IRIS
op_collection_id ftunivsalento
language English
topic Fluorescence
acridine orange
proton/dipeptide cotransport
intestine
(eel)
spellingShingle Fluorescence
acridine orange
proton/dipeptide cotransport
intestine
(eel)
VERRI, Tiziano
MAFFIA, Michele
STORELLI, Carlo
H+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the pH-sensitive dye acridine orange
topic_facet Fluorescence
acridine orange
proton/dipeptide cotransport
intestine
(eel)
description Monitoring the fluorescence quenching of the pH-sensitive dye Acridine orange, proton accumulation in the presence of an inside-negative transmembrane potential was measured in eel (Anguilla anguilla) intestinal brush-border membrane vesicles. It was demonstrated that the proton accumulation was specifically increased by the presence of the dipeptide glycyl-glycine in the extravesicular space, showing saturation kinetics at increasing dipeptide concentrations and was specifically inhibited by diethylpyrocarbonate. Data reported sugge
author2 Verri, Tiziano
Maffia, Michele
Storelli, Carlo
format Article in Journal/Newspaper
author VERRI, Tiziano
MAFFIA, Michele
STORELLI, Carlo
author_facet VERRI, Tiziano
MAFFIA, Michele
STORELLI, Carlo
author_sort VERRI, Tiziano
title H+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the pH-sensitive dye acridine orange
title_short H+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the pH-sensitive dye acridine orange
title_full H+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the pH-sensitive dye acridine orange
title_fullStr H+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the pH-sensitive dye acridine orange
title_full_unstemmed H+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the pH-sensitive dye acridine orange
title_sort h+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the ph-sensitive dye acridine orange
publishDate 1992
url http://hdl.handle.net/11587/108598
https://doi.org/10.1016/0005-2736(92)90303-4
http://www.sciencedirect.com/science/article/pii/0005273692903034
genre Anguilla anguilla
genre_facet Anguilla anguilla
op_relation info:eu-repo/semantics/altIdentifier/wos/WOS:A1992JQ43000017
volume:1110
firstpage:123
lastpage:126
numberofpages:4
journal:BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
http://hdl.handle.net/11587/108598
doi:10.1016/0005-2736(92)90303-4
info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0026672922
http://www.sciencedirect.com/science/article/pii/0005273692903034
op_doi https://doi.org/10.1016/0005-2736(92)90303-4
container_title Biochimica et Biophysica Acta (BBA) - Biomembranes
container_volume 1110
container_issue 1
container_start_page 123
op_container_end_page 126
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