H+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the pH-sensitive dye acridine orange
Monitoring the fluorescence quenching of the pH-sensitive dye Acridine orange, proton accumulation in the presence of an inside-negative transmembrane potential was measured in eel (Anguilla anguilla) intestinal brush-border membrane vesicles. It was demonstrated that the proton accumulation was spe...
| Published in: | Biochimica et Biophysica Acta (BBA) - Biomembranes |
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| Main Authors: | , , |
| Other Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
1992
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| Subjects: | |
| Online Access: | http://hdl.handle.net/11587/108598 https://doi.org/10.1016/0005-2736(92)90303-4 http://www.sciencedirect.com/science/article/pii/0005273692903034 |
| Summary: | Monitoring the fluorescence quenching of the pH-sensitive dye Acridine orange, proton accumulation in the presence of an inside-negative transmembrane potential was measured in eel (Anguilla anguilla) intestinal brush-border membrane vesicles. It was demonstrated that the proton accumulation was specifically increased by the presence of the dipeptide glycyl-glycine in the extravesicular space, showing saturation kinetics at increasing dipeptide concentrations and was specifically inhibited by diethylpyrocarbonate. Data reported sugge |
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