Comparative immunological characterization of type-specific and conserved B-cell epitopes of pinniped, felid and canid herpesviruses.

• Published in: Archives of Virology
• Main Authors: Lebich, M., Harder, T.C. (Timm), Frey, H.R., Visser, I.K.G. (Ilona), Osterhaus, A.D.M.E. (Albert), Liess, B.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 1994
• Subjects: 0 (Antibodies; Monoclonal); Viral); 0 (Epitopes); Alphaherpesvirinae/*immunology/isolation & purification; Animals; Antibodies; Monoclonal/immunology; Viral/immunology; Antibody Specificity; B-Lymphocytes/*immunology; Cats; Cells; Cultured; Comparative Study; Dogs; Epitopes/*immunology; Herpesviridae Infections/microbiology/veterinary; Immunoenzyme Techniques/veterinary; Neutralization Tests; Seals; Support; Non-U.S. Gov't; Swine; Phoca vitulina
• Online Access: http://repub.eur.nl/pub/3501; https://doi.org/10.1007/BF01321062

Murine monoclonal antibodies (MAbs) were generated against phocid herpesviruses (PhHV 2557/Han88 and 7848/Han90) isolated from European harbour seals (Phoca vitulina), and against strains of both felid (FHV strain FVR 605) and canid herpesviruses (CHV isolate 5105/Han89). MAbs were characterized with respect to certain biological properties and used to outline antigenicity profiles of isolates of PhHV (n = 8), FHV (n = 7) and CHV (n = 3) in enzyme immunoassays employing fixed infected ce