Analysis of the effect of microgravity on protein crystal quality: the case of a myoglobin triple mutant

• Published in: Acta Crystallographica Section D Biological Crystallography
• Main Authors: MIELE, Adriana Erica, BRUNORI, Maurizio, VALLONE, Beatrice, Luca Federici, Giuliano Sciara, Federica Draghi
• Other Authors: Miele, Adriana Erica, Luca, Federici, Giuliano, Sciara, Federica, Draghi, Brunori, Maurizio, Vallone, Beatrice
• Format: Article in Journal/Newspaper
• Language: English
• Published: BLACKWELL MUNKSGAARD 2003
• Subjects: atomic resolution crystal structure; crystal packing in microgravity; stereochemistry; structure-function relationship; Sperm whale
• Online Access: http://hdl.handle.net/11573/254456; https://doi.org/10.1107/s0907444903005924; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000183043700004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a; http://www.scopus.com/inward/record.url?eid=2-s2.0-0038384462&partnerID=65&md5=65ae42bee814658e1fae31b9cb238f42

Crystals of the Met derivative of the sperm whale myoglobin triple mutant Mb-YQR [L(B10)Y, H(E7)Q and T(E10)R] were grown under microgravity conditions and on earth by vapour diffusion. A comparison of crystal quality after complete data collection and processing shows how microgravity-grown crystals diffract to better resolution and lead to considerably improved statistics for X-ray diffraction data compared with crystals grown on earth under the same conditions. The same set of experiments was reproduced on two different Spacelab missions (ISS 6A and ISS 8A) in 2001 and 2002. The structure of this mutant myoglobin, refined using data collected at ELETTRA (Trieste, Italy) from both kinds of crystals, shows that X-ray diffraction from microgravity-grown crystals leads to better defined electron-density maps as well as improved geometrical quality of the refined model. Improvement of the stereochemical parameters of a protein structure is fundamental to quantitative analysis of its function and dynamics and hence to thorough understanding of the molecular mechanisms of action.