Characterization and tissue distribution of pyruvate carboxylase in atlantic salmon (Salmo salar)
1. 1. The mitochondrial fraction of liver from Atlantic salmon contained about 90% of subcellular pyruvate carboxylase activity (EC 6.4.1.1). 2. 2. Kidney, brain, heart, and gills contained 20, 19, 3 and 2%, respectively, of the activity observed per unit weight of liver. 3. 3. Assays with partially...
Published in: | Comparative Biochemistry and Physiology Part B: Comparative Biochemistry |
---|---|
Main Authors: | , , |
Format: | Text |
Language: | unknown |
Published: |
DigitalCommons@URI
1993
|
Subjects: | |
Online Access: | https://digitalcommons.uri.edu/favs_facpubs/139 https://doi.org/10.1016/0305-0491(93)90135-R |
Summary: | 1. 1. The mitochondrial fraction of liver from Atlantic salmon contained about 90% of subcellular pyruvate carboxylase activity (EC 6.4.1.1). 2. 2. Kidney, brain, heart, and gills contained 20, 19, 3 and 2%, respectively, of the activity observed per unit weight of liver. 3. 3. Assays with partially purified enzyme showed the level of acetyl CoA and the ratio of ATP/ADP to be the major regulators, with ADP (but not AMP) being a potent inhibitor of ATP binding. © 1993. |
---|