Characterization and tissue distribution of pyruvate carboxylase in atlantic salmon (Salmo salar)

1. 1. The mitochondrial fraction of liver from Atlantic salmon contained about 90% of subcellular pyruvate carboxylase activity (EC 6.4.1.1). 2. 2. Kidney, brain, heart, and gills contained 20, 19, 3 and 2%, respectively, of the activity observed per unit weight of liver. 3. 3. Assays with partially...

Full description

Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Comparative Biochemistry
Main Authors: Ji, Hong, Bradley, Terence M., Tremblay, George C.
Format: Text
Language:unknown
Published: DigitalCommons@URI 1993
Subjects:
Atlantic salmon
Salmo salar
Online Access:https://digitalcommons.uri.edu/favs_facpubs/139
https://doi.org/10.1016/0305-0491(93)90135-R
Description
Summary:1. 1. The mitochondrial fraction of liver from Atlantic salmon contained about 90% of subcellular pyruvate carboxylase activity (EC 6.4.1.1). 2. 2. Kidney, brain, heart, and gills contained 20, 19, 3 and 2%, respectively, of the activity observed per unit weight of liver. 3. 3. Assays with partially purified enzyme showed the level of acetyl CoA and the ratio of ATP/ADP to be the major regulators, with ADP (but not AMP) being a potent inhibitor of ATP binding. © 1993.

Similar Items