Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of the sGnRH promoter of Atlantic salmon

• Published in: Journal of Molecular Endocrinology
• Main Authors: Uzbekova, Svetlana, Chyb, J., Ferriere, F., Bailhache, T., Prunet, Patrick, Alestrom, P., Breton, Bernard
• Other Authors: Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Interactions cellulaires et moléculaires (ICM), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Norwegian School of Veterinary Science, Norvège
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2000
• Subjects: SALMONIDAE ONCORHYNCHUS MYKISS; [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism; Atlantic salmon; Salmo salar
• Online Access: https://hal.inrae.fr/hal-02698389; https://doi.org/10.1677/jme.0.0250337

International audience A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals that had integrated one copy of the transgene into their genome and transmitted it through the germline. Antisense-sGnRH mRNA (AS) was expressed mainly in the brain of transgenic AS(+) fish. Levels of sGnRH endogenous mRNA in the brain were lower in 11-month-old AS(+) fish compared with nontransgenic AS(-) individuals from the same F2 progeny, sGnRH levels significantly decreased in the pituitary of transgenic males and females around the maturation period and in the brain of AS(+) immature females compared with controls. No reliable statistical difference was found in the levels of FSH and LH between AS(+) and AS(-) groups either in immature or mature fish. The majority of transgenic fish reached maturity at the same time as did nontransgenic individuals, although the maturation of AS(+) animals seemed to be more asynchronous. For the first time, the influence of antisense messengers on endogenous mRNA in transgenic fish and the corresponding protein is described.