Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of the sGnRH promoter of Atlantic salmon
International audience A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals tha...
Published in: | Journal of Molecular Endocrinology |
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Online Access: | https://hal.inrae.fr/hal-02698389 https://doi.org/10.1677/jme.0.0250337 |
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ftunivrennes1hal:oai:HAL:hal-02698389v1 2023-06-11T04:10:17+02:00 Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of the sGnRH promoter of Atlantic salmon Uzbekova, Svetlana Chyb, J. Ferriere, F. Bailhache, T. Prunet, Patrick Alestrom, P. Breton, Bernard Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) Interactions cellulaires et moléculaires (ICM) Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) Norwegian School of Veterinary Science Norvège 2000 https://hal.inrae.fr/hal-02698389 https://doi.org/10.1677/jme.0.0250337 en eng HAL CCSD BioScientifica info:eu-repo/semantics/altIdentifier/doi/10.1677/jme.0.0250337 info:eu-repo/semantics/altIdentifier/pmid/11116212 hal-02698389 https://hal.inrae.fr/hal-02698389 doi:10.1677/jme.0.0250337 PRODINRA: 55799 PUBMED: 11116212 WOS: 000167621000008 ISSN: 0952-5041 EISSN: 1479-6813 Journal of Molecular Endocrinology https://hal.inrae.fr/hal-02698389 Journal of Molecular Endocrinology, 2000, 25, pp.337-350. ⟨10.1677/jme.0.0250337⟩ SALMONIDAE ONCORHYNCHUS MYKISS [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism info:eu-repo/semantics/article Journal articles 2000 ftunivrennes1hal https://doi.org/10.1677/jme.0.0250337 2023-05-30T22:51:40Z International audience A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals that had integrated one copy of the transgene into their genome and transmitted it through the germline. Antisense-sGnRH mRNA (AS) was expressed mainly in the brain of transgenic AS(+) fish. Levels of sGnRH endogenous mRNA in the brain were lower in 11-month-old AS(+) fish compared with nontransgenic AS(-) individuals from the same F2 progeny, sGnRH levels significantly decreased in the pituitary of transgenic males and females around the maturation period and in the brain of AS(+) immature females compared with controls. No reliable statistical difference was found in the levels of FSH and LH between AS(+) and AS(-) groups either in immature or mature fish. The majority of transgenic fish reached maturity at the same time as did nontransgenic individuals, although the maturation of AS(+) animals seemed to be more asynchronous. For the first time, the influence of antisense messengers on endogenous mRNA in transgenic fish and the corresponding protein is described. Article in Journal/Newspaper Atlantic salmon Salmo salar Université de Rennes 1: Publications scientifiques (HAL) Journal of Molecular Endocrinology 25 3 337 350 |
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Open Polar |
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Université de Rennes 1: Publications scientifiques (HAL) |
op_collection_id |
ftunivrennes1hal |
language |
English |
topic |
SALMONIDAE ONCORHYNCHUS MYKISS [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism |
spellingShingle |
SALMONIDAE ONCORHYNCHUS MYKISS [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism Uzbekova, Svetlana Chyb, J. Ferriere, F. Bailhache, T. Prunet, Patrick Alestrom, P. Breton, Bernard Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of the sGnRH promoter of Atlantic salmon |
topic_facet |
SALMONIDAE ONCORHYNCHUS MYKISS [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism |
description |
International audience A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals that had integrated one copy of the transgene into their genome and transmitted it through the germline. Antisense-sGnRH mRNA (AS) was expressed mainly in the brain of transgenic AS(+) fish. Levels of sGnRH endogenous mRNA in the brain were lower in 11-month-old AS(+) fish compared with nontransgenic AS(-) individuals from the same F2 progeny, sGnRH levels significantly decreased in the pituitary of transgenic males and females around the maturation period and in the brain of AS(+) immature females compared with controls. No reliable statistical difference was found in the levels of FSH and LH between AS(+) and AS(-) groups either in immature or mature fish. The majority of transgenic fish reached maturity at the same time as did nontransgenic individuals, although the maturation of AS(+) animals seemed to be more asynchronous. For the first time, the influence of antisense messengers on endogenous mRNA in transgenic fish and the corresponding protein is described. |
author2 |
Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) Interactions cellulaires et moléculaires (ICM) Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS) Norwegian School of Veterinary Science Norvège |
format |
Article in Journal/Newspaper |
author |
Uzbekova, Svetlana Chyb, J. Ferriere, F. Bailhache, T. Prunet, Patrick Alestrom, P. Breton, Bernard |
author_facet |
Uzbekova, Svetlana Chyb, J. Ferriere, F. Bailhache, T. Prunet, Patrick Alestrom, P. Breton, Bernard |
author_sort |
Uzbekova, Svetlana |
title |
Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of the sGnRH promoter of Atlantic salmon |
title_short |
Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of the sGnRH promoter of Atlantic salmon |
title_full |
Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of the sGnRH promoter of Atlantic salmon |
title_fullStr |
Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of the sGnRH promoter of Atlantic salmon |
title_full_unstemmed |
Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of the sGnRH promoter of Atlantic salmon |
title_sort |
transgenic rainbow trout expressed sgnrh-antisense rna under the control of the sgnrh promoter of atlantic salmon |
publisher |
HAL CCSD |
publishDate |
2000 |
url |
https://hal.inrae.fr/hal-02698389 https://doi.org/10.1677/jme.0.0250337 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ISSN: 0952-5041 EISSN: 1479-6813 Journal of Molecular Endocrinology https://hal.inrae.fr/hal-02698389 Journal of Molecular Endocrinology, 2000, 25, pp.337-350. ⟨10.1677/jme.0.0250337⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1677/jme.0.0250337 info:eu-repo/semantics/altIdentifier/pmid/11116212 hal-02698389 https://hal.inrae.fr/hal-02698389 doi:10.1677/jme.0.0250337 PRODINRA: 55799 PUBMED: 11116212 WOS: 000167621000008 |
op_doi |
https://doi.org/10.1677/jme.0.0250337 |
container_title |
Journal of Molecular Endocrinology |
container_volume |
25 |
container_issue |
3 |
container_start_page |
337 |
op_container_end_page |
350 |
_version_ |
1768384597313191936 |