Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of the sGnRH promoter of Atlantic salmon

International audience A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals tha...

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Bibliographic Details
Published in:Journal of Molecular Endocrinology
Main Authors: Uzbekova, Svetlana, Chyb, J., Ferriere, F., Bailhache, T., Prunet, Patrick, Alestrom, P., Breton, Bernard
Other Authors: Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Interactions cellulaires et moléculaires (ICM), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Norwegian School of Veterinary Science, Norvège
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2000
Subjects:
SALMONIDAE ONCORHYNCHUS MYKISS
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
Atlantic salmon
Salmo salar
Online Access:https://hal.inrae.fr/hal-02698389
https://doi.org/10.1677/jme.0.0250337
Description
Summary:International audience A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals that had integrated one copy of the transgene into their genome and transmitted it through the germline. Antisense-sGnRH mRNA (AS) was expressed mainly in the brain of transgenic AS(+) fish. Levels of sGnRH endogenous mRNA in the brain were lower in 11-month-old AS(+) fish compared with nontransgenic AS(-) individuals from the same F2 progeny, sGnRH levels significantly decreased in the pituitary of transgenic males and females around the maturation period and in the brain of AS(+) immature females compared with controls. No reliable statistical difference was found in the levels of FSH and LH between AS(+) and AS(-) groups either in immature or mature fish. The majority of transgenic fish reached maturity at the same time as did nontransgenic individuals, although the maturation of AS(+) animals seemed to be more asynchronous. For the first time, the influence of antisense messengers on endogenous mRNA in transgenic fish and the corresponding protein is described.

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