Molecular diversity of the rumen microbiome of norwegian reindeer on natural summer pasture

The molecular diversity of the rumen microbiome was investigated in five semi-domesticated adult female Norwegian reindeer (Rangifer tarandus tarandus) grazing on natural summer pastures on the coast of northern Norway (71.00° N, 25.30° E). Mean population densities (numbers per gram wet weight) of...

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Bibliographic Details
Published in:Microbial Ecology
Main Authors: Sundset, Monica A., Edwards, Joan E., Cheng, Yan Fen, Senosiain, Roberto S., Fraile, Maria N., Northwood, Korinne S., Præsteng, Kirsti E., Glad, Trine, Mathiesen, Svein D., Wright, André-Denis G.
Format: Article in Journal/Newspaper
Language:English
Published: Springer Science and Business Media LLC 2009
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Online Access:https://espace.library.uq.edu.au/view/UQ:def05fc
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Summary:The molecular diversity of the rumen microbiome was investigated in five semi-domesticated adult female Norwegian reindeer (Rangifer tarandus tarandus) grazing on natural summer pastures on the coast of northern Norway (71.00° N, 25.30° E). Mean population densities (numbers per gram wet weight) of methanogenic archaea, rumen bacteria and ciliate protozoa, estimated using quantitative real-time polymerase chain reaction (PCR), were 3.17∈×∈10, 5.17∈×∈10 and 4.02∈×∈10, respectively. Molecular diversity of rumen methanogens was revealed using a 16S rRNA gene library (54 clones) constructed using pooled PCR products from the whole rumen contents of the five individual reindeer. Based upon a similarity criterion of