Genetic identification of Southern Ocean octopod samples using mtCOI

East Antarctic octopods were identified by sequencing mtCOI and using four analytical approaches: Neighbor-joining by Kimura-2-Parameter-based distances, character-based, BLAST, and Bayesian Inference of Phylogeny. Although the distance-based analytical approaches identified a high proportion of the...

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Bibliographic Details
Published in:Comptes Rendus Biologies
Main Authors: Undheim, Eivind Andreas Baste, Norman, Janette Ann, Thoen, Hanne Halkinrud, Fry, Bryan Grieg
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier Masson 2010
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Online Access:https://espace.library.uq.edu.au/view/UQ:310934
Description
Summary:East Antarctic octopods were identified by sequencing mtCOI and using four analytical approaches: Neighbor-joining by Kimura-2-Parameter-based distances, character-based, BLAST, and Bayesian Inference of Phylogeny. Although the distance-based analytical approaches identified a high proportion of the sequences (99.5% to genus and 88.1% to species level), these results are undermined by the absence of a clear gap between intra-and interspecific variation. The character-based approach gave highly conflicting results compared to the distance-based methods and failed to identify apomorphic characters for many of the species. While a DNA independent approach is necessary for validation of the method comparisons, crude morphological observations give early support to the distance-based results and indicate extensive range expansions of several species compared to previous studies. Furthermore, the use of distance-based phylogenetic methods nevertheless group specimens into plausible species clades that are highly useful in non-taxonomical or non-systematic studies. (C) 2010 Academie des sciences. Published by Elsevier Masson SAS. All rights reserved.