Cytochrome P450 isozyme protein verified in the skin of southern hemisphere humpback whales (Megaptera novaeangliae): Implications for biochemical biomarker assessment

Large mysticete whales represent a unique challenge for chemical risk assessment. Few epidemiological investigations are possible due to the low incidence of adult stranding events. Similarly their often extreme life-history adaptations of prolonged migration and fasting challenge exposure assumptio...

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Bibliographic Details
Published in:Marine Pollution Bulletin
Main Authors: Waugh, Courtney A., Huston, Wilhelmina M., Noad, Michael J., Bengtson Nash, Susan
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2011
Subjects:
Humpback whale
CYP1A1
Biomarkers
Western blot
Phoca-vitulina
Expression
Induction
Exposure
Biopsy
P4501A1
Cetaceans
Receptor
Beluga
1104 Aquatic Science
1910 Oceanography
2310 Pollution
Beluga*
Humpback Whale
Megaptera novaeangliae
Phoca vitulina
Online Access:https://espace.library.uq.edu.au/view/UQ:267982
Description
Summary:Large mysticete whales represent a unique challenge for chemical risk assessment. Few epidemiological investigations are possible due to the low incidence of adult stranding events. Similarly their often extreme life-history adaptations of prolonged migration and fasting challenge exposure assumptions. Molecular biomarkers offer the potential to complement information yielded through tissue chemical analysis, as well as providing evidence of a molecular response to chemical exposure. In this study we confirm the presence of cytochrome P450 reductase (CPR) and cytochrome P450 isoenzyme 1A1 (CYP1A1) in epidermal tissue of southern hemisphere humpback whales (Megaptera novaeangliae). The detection of CYP1A1 in the integument of the humpback whale affords the opportunity for further quantitative non-destructive investigations of enzyme activity as a function of chemical stress. (C) 2011 Elsevier Ltd. All rights reserved.

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