Genetic variability and evolutionary analyses of the coat protein gene of Tomato mosaic virus

• Published in: Virus Genes
• Main Authors: Rangel, Ezequiel, Alfaro Fernández, Ana Olvido, Font San Ambrosio, Maria Isabel, Luis-Arteaga, M., Rubio, Luis
• Other Authors: Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani, Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural, Instituto Nacional de Investigaciones Agrícolas, Venezuela
• Format: Article in Journal/Newspaper
• Language: English
• Published: Springer Verlag (Germany) 2011
• Subjects: Phylogeny; Plant virus; Selection; Tobamovirus; ToMV; Water; Article; Aubergine; Bootstrapping; Bottleneck population; Brazil; China; Cladistics; Coat protein gene; Controlled study; Cornus; Deglaciation; Denmark; Gene structure; Genetic stability; Genetic variability; Genotype; Geographic distribution; Germany; Higher plant; Iran; Kazakhstan; Korea; Lake; Lilac; Malaysia; Molecular evolution; Nonhuman; Nucleotide sequence; Pepper; Priority journal; Purifying selection; Red spruce; Sequence analysis; Sequence homology; Spain; Stream (river); Taiwan; Tea; Tomato; Tomato mosaic virus; United States; Virus gene; Virus isolation; Capsid Proteins; Alfaro; Greenland; Ice Lakes; Navarro; Rubio
• Online Access: http://hdl.handle.net/10251/83102; https://doi.org/10.1007/s11262-011-0651-3

[EN] Tomato mosaic virus (ToMV), a member of the genus Tobamovirus, infects several ornamental and horticultural crops worldwide. In this study, the nucleotide sequences of the coat protein gene of worldwide ToMV isolates were analyzed to estimate the genetic structure and diversity of this virus and the involved evolutionary forces. The phylogenetic analysis showed three clades with high bootstrap support: Clade I contained three ToMV isolates from Brazil collected from pepper, Clade II comprised one Brazilian ToMV isolate from pepper, and Clade III was composed of ToMV isolates collected from different plant hosts (pepper, tomato, eggplant, lilac, camellia, dogwood, red spruce, etc.) and water (from melting ice, lakes and streams) from different countries: USA, Brazil, Korea, Germany, Spain, Denmark (Greenland), China, Taiwan, Malaysia, Iran, and Kazakhstan. With the exception of Brazil, nucleotide diversity within and between different geographic regions was very low, although statistical analyses suggested some gene flow between most of these regions. Our analyses also suggested a strong negative selection which could have contributed to the genetic stability of ToMV. © Springer Science+Business Media, LLC 2011. E. A. Rangel was the recipient of a predoctoral fellowship from the Instituto Nacional de Investigaciones Agricolas (INIA) from Venezuela. Rangel, E.; Alfaro Fernández, AO.; Font San Ambrosio, MI.; Luis-Arteaga, M.; Rubio, L. (2011). Genetic variability and evolutionary analyses of the coat protein gene of Tomato mosaic virus. Virus Genes. 43(3):435-438. doi:10.1007/s11262-011-0651-3 S 435 438 43 3 J. Holland, K. Spindler, F. Horodyski, E. Grabau, S. Nichol, S. VandePol, Science 215, 1577–1585 (1982) E.R. Chare, E.C. Holmes, Arch. Virol. 151, 933–948 (1996) A. Moya, E.C. Holmes, F. Gónzalez-Candelas, Nat. Rev. Microbiol. 2, 279–288 (2004) F. García-Arenal, A. Fraile, J.M. Malpica, Annu. Rev. Phytopathol. 39, 157–186 (2001) M.C. Vives, L. Rubio, L. Galipienso, L. Navarro, P. ...