Sodium affects the sperm motility in the European eel

• Published in: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
• Main Authors: Vilchez Olivencia, Maria Carmen, MORINI, MARINA, Peñaranda, D.S., Gallego Albiach, Victor, Asturiano Nemesio, Juan Francisco, Pérez Igualada, Luz María
• Other Authors: Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal, Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural, Generalitat Valenciana, European Cooperation in Science and Technology, Universitat Politècnica de València
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2016
• Subjects: Anguilla anguilla; Ion channels; Flow cytometry; Calibration; CASA; ASMA; Cell volume; Monensin; Amiloride; Intracellular sodium; BIOLOGIA ANIMAL; PRODUCCION ANIMAL; Nemesio; European eel
• Online Access: http://hdl.handle.net/10251/80574; https://doi.org/10.1016/j.cbpa.2016.04.008

[EN] The role of seminal plasma sodium and activation media sodium on sperm motility was examined by selectively removing the element from these two media, in European eel sperm. Sperm size (sperm head area) was also measured using an ASMA (Automated Sperm Morphometry Analyses) system, in the different conditions. Intracellular sodium [Na+](i) was quantitatively analyzed by first time in the spermatozoa from a marine fish species. Measurement of [Na+](i) was done before and after motility activation, by Flow Cytometry, using CoroNa Green AM as a dye. Sperm motility activation induced an increase in [Na+](i) from 96.72 mM in quiescent stage to 152.21 mM post-activation in seawater. A significant decrease in sperm head area was observed post activation in seawater. There was a notable reduction in sperm motility when sodium was removed from the seminal plasma, but not when it was removed from the activation media. Sodium removal was also linked to a significant reduction in sperm head area in comparison to the controls. Our results indicate that the presence of the ion Na+ in the seminal plasma (or in the extender medium) is necessary for the preservation of sperm motility in European eel, probably because it plays a role in maintaining an appropriate sperm cell volume in the quiescent stage of the spermatozoa. (C) 2016 Elsevier Inc All rights reserved. Funded from the SPERMOT project (Spanish Ministry of Science and Innovation, MICINN; AGL2010-16009). M.C. Vilchez has a predoctoral grant from UPV PAID Program (2011-S2-02-6521), Marina Morini has a predoctoral grant from Generalitat Valenciana (Programa Grisolia, GRISOLIA/2012/006), Victor Gallego has a postdoctoral contract from UPV (PAID-10-14), and David S. Penaranda was supported by MICINN (PTA2011-4948-I) and UPV (PTA2011-4948-I). Grants to attend meetings were received from COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE). Vilchez Olivencia, MC.; Morini, M.; Peñaranda, D.; Gallego Albiach, V.; Asturiano Nemesio, JF.; Pérez Igualada, LM. ...