Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla)

• Published in: Fish Physiology and Biochemistry
• Main Authors: Blanes-García, Marta, Marinovic, Zoran, Herranz-Jusdado, Juan Germán, Xie, Xuan, Ferrao, Leonor, Gallego Albiach, Victor, Pérez Igualada, Luz María, Baloch, Abdul Rasheed, Horváth, Ákos, Psenicka, Martin, Asturiano, Juan F., Morini, Marina
• Other Authors: Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural, European Commission, Generalitat Valenciana, Agencia Estatal de Investigación, Grant Agency of the Czech Republic, Ministerio de Ciencia e Innovación, Universitat Politècnica de València, Ministry of Innovation and Technology, Hungría, Ministry of Education, Youth and Sport of the Czech Republic
• Format: Article in Journal/Newspaper
• Language: English
• Published: Springer-Verlag 2024
• Subjects: Testis; Fish; Gene expression; Vasa; Dnd1; Nanos2; PRODUCCION ANIMAL; Anguilla anguilla
• Online Access: http://hdl.handle.net/10251/206520; https://doi.org/10.1007/s10695-024-01338-1

[EN] Identification of specific molecular markers for spermatogonial stem cells in teleost is crucial for enhancing the efficacy of reproductive biotechnologies in aquaculture, such as transplantation and surrogate production in fishes. Since it is not yet possible to distinguish spermatogonial stem cells of European eel (Anguilla anguilla) using specific molecular markers, we isolated spermatogonial cells from immature European eels to find these potential markers. We attempted this by studying three candidate genes: vasa, nanos2, and dnd1. Two vasa (vasa1 and vasa2) genes, nanos2, and dnd1 were identified, characterized, and studied in the muscle, testis, and isolated spermatogonia. Our results showed that vasa1 and vasa2 had the highest levels of expression when measured by qPCR. In situ hybridization and immunochemistry assays showed that the four genes were localized explicitly in type A spermatogonia. However, vasa1 and vasa2 exhibited stronger signals in the immature testicular tissue than the other two potential markers. According to this, vasa1 and vasa2 were found to be the most effective markers for spermatogonial cells in the European eel. Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Funded by the European Union s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement Nº 642893 (IMPRESS; including the JGHJ and ARB predoctoral contracts), and by the Ministerio de Ciencia e Innovación y Universidades (Project EELGONIA; RTI2018-096413-BI00). MBG and LF have PhD contracts from the UPV (PAID01 20) and GVA (GRISOLIAP/2020/063), respectively. VG joined a grant RYC2021-031558-I funded by MCIN/AEI/10.13039/501100011033, and by European Union NextGenerationEU/PRTR. MM has a postdoc contract supported byMCIN/AEI/10.13039/501100011033 funded by European Union NextGenerationEU/PRTR (C17.I1) and by Generalitat Valenciana (THINKINAZUL/2021/012) to SEASPERM. MP was fnancially supported by the Ministry of Education, Youth and Sports of ...