Characterisation of European eel (Anguilla anguilla) spermatozoa morphometry using Trumorph tool in fixed and non-fixed samples
[EN] Currently, the techniques used to analyse fish spermatozoa head morphology are not only subjective and timeconsuming, but also, variable due to alteration of real spermatozoa size during sample manipulation. Thereby, it is important to develop a new method to obtain accurate and objective resul...
Published in: | Aquaculture |
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Main Authors: | , , , , , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2022
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Subjects: | |
Online Access: | http://hdl.handle.net/10251/193396 https://doi.org/10.1016/j.aquaculture.2022.738047 |
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ftunivpvalencia:oai:riunet.upv.es:10251/193396 |
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openpolar |
institution |
Open Polar |
collection |
Politechnical University of Valencia: RiuNet |
op_collection_id |
ftunivpvalencia |
language |
English |
topic |
CASA-Morph system Sperm morphometry characterisation Fixation solution TruMorph® tool Pressure Realistic measurements PRODUCCION ANIMAL 14.- Conservar y utilizar de forma sostenible los océanos mares y recursos marinos para lograr el desarrollo sostenible |
spellingShingle |
CASA-Morph system Sperm morphometry characterisation Fixation solution TruMorph® tool Pressure Realistic measurements PRODUCCION ANIMAL 14.- Conservar y utilizar de forma sostenible los océanos mares y recursos marinos para lograr el desarrollo sostenible Caldeira, Carina Hernández-Ibánez, Sandra Vendrell, Alberto Valverde, Anthony Garcia-Molina, Almudena Gallego Albiach, Victor Asturiano, Juan F. Soler, Carles Characterisation of European eel (Anguilla anguilla) spermatozoa morphometry using Trumorph tool in fixed and non-fixed samples |
topic_facet |
CASA-Morph system Sperm morphometry characterisation Fixation solution TruMorph® tool Pressure Realistic measurements PRODUCCION ANIMAL 14.- Conservar y utilizar de forma sostenible los océanos mares y recursos marinos para lograr el desarrollo sostenible |
description |
[EN] Currently, the techniques used to analyse fish spermatozoa head morphology are not only subjective and timeconsuming, but also, variable due to alteration of real spermatozoa size during sample manipulation. Thereby, it is important to develop a new method to obtain accurate and objective results. So far, computer-assisted systems for morphometry analysis were developed and validated for mammals, although they could also be adapted to fish spermatozoa. The present study aimed to characterise the European eel spermatozoa morphometry, comparing the measurements (area and perimeter) obtained by computer-assisted spermatozoa between pretreated or non-treated eel sperm samples (n = 5) with fixation solution. In both protocols, the TruMorph (R) tool was used to apply a constant force to extend the cells in a thin layer. Images of spermatozoa were captured using a 40x negative phase contrast objective and analysed with the ISASv1 CASA-Morph system. Sperm head morphometry showed significant differences in area and perimeter comparing both protocols. Besides, the head size analysis using TruMorph (R) without fixation along time showed that the sperm membrane remains intact with the use of this technique, preserving the semipermeable condition. Considering the fact that fixation solution produces dehydration/hydration that could affect the real spermatozoa size, the simple use of TruMorph (R) without fixation combined with CASA-Morph analysis could allow more realistic measurements of eel spermatozoa head. This project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement N. 642893 (IMPRESS). AV is granted by the CONICIT and MICITT, Costa Rica. VG has a post-doc contract from the Spanish Ministry of Innovation, Science and Universities (Juan de la Cierva-Incorporaci ' on; IJCI-2017-34200). Caldeira, C.; Hernández-Ibánez, S.; Vendrell, A.; Valverde, A.; Garcia-Molina, A.; Gallego Albiach, V.; Asturiano, JF. (2022). ... |
author2 |
Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural COMISION DE LAS COMUNIDADES EUROPEA Ministerio de Ciencia, Innovación y Universidades Ministerio de Ciencia Tecnología y Telecomunicaciones de Costa Rica Consejo Nacional para Investigaciones Científicas y Tecnológicas, Costa Rica |
format |
Article in Journal/Newspaper |
author |
Caldeira, Carina Hernández-Ibánez, Sandra Vendrell, Alberto Valverde, Anthony Garcia-Molina, Almudena Gallego Albiach, Victor Asturiano, Juan F. Soler, Carles |
author_facet |
Caldeira, Carina Hernández-Ibánez, Sandra Vendrell, Alberto Valverde, Anthony Garcia-Molina, Almudena Gallego Albiach, Victor Asturiano, Juan F. Soler, Carles |
author_sort |
Caldeira, Carina |
title |
Characterisation of European eel (Anguilla anguilla) spermatozoa morphometry using Trumorph tool in fixed and non-fixed samples |
title_short |
Characterisation of European eel (Anguilla anguilla) spermatozoa morphometry using Trumorph tool in fixed and non-fixed samples |
title_full |
Characterisation of European eel (Anguilla anguilla) spermatozoa morphometry using Trumorph tool in fixed and non-fixed samples |
title_fullStr |
Characterisation of European eel (Anguilla anguilla) spermatozoa morphometry using Trumorph tool in fixed and non-fixed samples |
title_full_unstemmed |
Characterisation of European eel (Anguilla anguilla) spermatozoa morphometry using Trumorph tool in fixed and non-fixed samples |
title_sort |
characterisation of european eel (anguilla anguilla) spermatozoa morphometry using trumorph tool in fixed and non-fixed samples |
publisher |
Elsevier |
publishDate |
2022 |
url |
http://hdl.handle.net/10251/193396 https://doi.org/10.1016/j.aquaculture.2022.738047 |
long_lat |
ENVELOPE(-60.873,-60.873,-64.156,-64.156) ENVELOPE(-62.017,-62.017,-64.017,-64.017) |
geographic |
Cierva Molina |
geographic_facet |
Cierva Molina |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
Aquaculture info:eu-repo/grantAgreement/EC/H2020/642893/EU info:eu-repo/grantAgreement/MCIU//IJCI-2017-34200//Ayuda Juan de la Cierva Incorporación - Gallego Albiach/ https://doi.org/10.1016/j.aquaculture.2022.738047 10.1093/humrep/17.2.375 10.1016/j.theriogenology.2006.02.041 10.1111/j.1439-0531.2006.00746.x 10.1007/BF00204873 10.1046/j.1365-2109.2000.00423.x 10.1111/j.1439-0531.1999.tb01387.x 10.1046/j.1439-0531.2001.00291.x 10.1016/j.aquaculture.2018.12.001 10.1093/humupd/4.1.73 10.1093/humrep/deh410 10.1016/S0015-0282(16)55686-6 10.1111/j.1439-0426.2010.01529.x 10.2164/jandrol.05122 10.1111/j.1095-8649.1998.tb01836.x 10.1071/RD17460 10.1111/raq.12253 10.1016/j.aquaculture.2012.04.041 10.1016/j.theriogenology.2011.10.010 10.1016/j.theriogenology.2013.01.019 10.1016/j.theriogenology.2014.05.026 10.1016/j.aquaculture.2013.10.022 10.1007/s10695-018-0505-1 10.4103/1008-682X.188448 10.1002/j.1939-4640.1995.tb01736.x 10.1016/S1532-0456(01)00270-8 10.17221/7188-CJAS 10.1016/j.theriogenology.2005.08.008 10.1111/j.1439-0426.2008.01135.x 10.1046/j.1463-6395.2003.00138.x 10.1016/j.anireprosci.2005.06.026 10.1111/j.1439-0426.2008.01124.x 10.1111/j.1439-0531.2008.01206.x 10.1111/j.1095-8649.2000.tb02227.x 10.1016/j.theriogenology.2004.01.005 10.1016/S0093-691X(98)00110-1 10.4103/1008-682X.188660 10.4103/1008-682X.188451 10.1046/j.1365-2605.2003.00422.x 10.1016/j.anireprosci.2015.04.001 10.1071/RD14087 10.1111/j.1439-0426.2008.01145.x 10.1046/j.1095-8649.2003.00226.x 10.1095/biolreprod66.1.98 10.1016/S0015-0282(16)54310-6 10.1016/j.anireprosci.2015.03.002 10.1093/humupd/3.3.205 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/embargoedAccess |
op_doi |
https://doi.org/10.1016/j.aquaculture.2022.738047 |
container_title |
Aquaculture |
container_volume |
553 |
container_start_page |
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ftunivpvalencia:oai:riunet.upv.es:10251/193396 2024-01-28T09:58:39+01:00 Characterisation of European eel (Anguilla anguilla) spermatozoa morphometry using Trumorph tool in fixed and non-fixed samples Caldeira, Carina Hernández-Ibánez, Sandra Vendrell, Alberto Valverde, Anthony Garcia-Molina, Almudena Gallego Albiach, Victor Asturiano, Juan F. Soler, Carles Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural COMISION DE LAS COMUNIDADES EUROPEA Ministerio de Ciencia, Innovación y Universidades Ministerio de Ciencia Tecnología y Telecomunicaciones de Costa Rica Consejo Nacional para Investigaciones Científicas y Tecnológicas, Costa Rica 2022-05-15 http://hdl.handle.net/10251/193396 https://doi.org/10.1016/j.aquaculture.2022.738047 eng eng Elsevier Aquaculture info:eu-repo/grantAgreement/EC/H2020/642893/EU info:eu-repo/grantAgreement/MCIU//IJCI-2017-34200//Ayuda Juan de la Cierva Incorporación - Gallego Albiach/ https://doi.org/10.1016/j.aquaculture.2022.738047 10.1093/humrep/17.2.375 10.1016/j.theriogenology.2006.02.041 10.1111/j.1439-0531.2006.00746.x 10.1007/BF00204873 10.1046/j.1365-2109.2000.00423.x 10.1111/j.1439-0531.1999.tb01387.x 10.1046/j.1439-0531.2001.00291.x 10.1016/j.aquaculture.2018.12.001 10.1093/humupd/4.1.73 10.1093/humrep/deh410 10.1016/S0015-0282(16)55686-6 10.1111/j.1439-0426.2010.01529.x 10.2164/jandrol.05122 10.1111/j.1095-8649.1998.tb01836.x 10.1071/RD17460 10.1111/raq.12253 10.1016/j.aquaculture.2012.04.041 10.1016/j.theriogenology.2011.10.010 10.1016/j.theriogenology.2013.01.019 10.1016/j.theriogenology.2014.05.026 10.1016/j.aquaculture.2013.10.022 10.1007/s10695-018-0505-1 10.4103/1008-682X.188448 10.1002/j.1939-4640.1995.tb01736.x 10.1016/S1532-0456(01)00270-8 10.17221/7188-CJAS 10.1016/j.theriogenology.2005.08.008 10.1111/j.1439-0426.2008.01135.x 10.1046/j.1463-6395.2003.00138.x 10.1016/j.anireprosci.2005.06.026 10.1111/j.1439-0426.2008.01124.x 10.1111/j.1439-0531.2008.01206.x 10.1111/j.1095-8649.2000.tb02227.x 10.1016/j.theriogenology.2004.01.005 10.1016/S0093-691X(98)00110-1 10.4103/1008-682X.188660 10.4103/1008-682X.188451 10.1046/j.1365-2605.2003.00422.x 10.1016/j.anireprosci.2015.04.001 10.1071/RD14087 10.1111/j.1439-0426.2008.01145.x 10.1046/j.1095-8649.2003.00226.x 10.1095/biolreprod66.1.98 10.1016/S0015-0282(16)54310-6 10.1016/j.anireprosci.2015.03.002 10.1093/humupd/3.3.205 http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/embargoedAccess CASA-Morph system Sperm morphometry characterisation Fixation solution TruMorph® tool Pressure Realistic measurements PRODUCCION ANIMAL 14.- Conservar y utilizar de forma sostenible los océanos mares y recursos marinos para lograr el desarrollo sostenible info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2022 ftunivpvalencia https://doi.org/10.1016/j.aquaculture.2022.738047 2024-01-03T00:07:09Z [EN] Currently, the techniques used to analyse fish spermatozoa head morphology are not only subjective and timeconsuming, but also, variable due to alteration of real spermatozoa size during sample manipulation. Thereby, it is important to develop a new method to obtain accurate and objective results. So far, computer-assisted systems for morphometry analysis were developed and validated for mammals, although they could also be adapted to fish spermatozoa. The present study aimed to characterise the European eel spermatozoa morphometry, comparing the measurements (area and perimeter) obtained by computer-assisted spermatozoa between pretreated or non-treated eel sperm samples (n = 5) with fixation solution. In both protocols, the TruMorph (R) tool was used to apply a constant force to extend the cells in a thin layer. Images of spermatozoa were captured using a 40x negative phase contrast objective and analysed with the ISASv1 CASA-Morph system. Sperm head morphometry showed significant differences in area and perimeter comparing both protocols. Besides, the head size analysis using TruMorph (R) without fixation along time showed that the sperm membrane remains intact with the use of this technique, preserving the semipermeable condition. Considering the fact that fixation solution produces dehydration/hydration that could affect the real spermatozoa size, the simple use of TruMorph (R) without fixation combined with CASA-Morph analysis could allow more realistic measurements of eel spermatozoa head. This project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement N. 642893 (IMPRESS). AV is granted by the CONICIT and MICITT, Costa Rica. VG has a post-doc contract from the Spanish Ministry of Innovation, Science and Universities (Juan de la Cierva-Incorporaci ' on; IJCI-2017-34200). Caldeira, C.; Hernández-Ibánez, S.; Vendrell, A.; Valverde, A.; Garcia-Molina, A.; Gallego Albiach, V.; Asturiano, JF. (2022). ... Article in Journal/Newspaper Anguilla anguilla Politechnical University of Valencia: RiuNet Cierva ENVELOPE(-60.873,-60.873,-64.156,-64.156) Molina ENVELOPE(-62.017,-62.017,-64.017,-64.017) Aquaculture 553 738047 |